GAP43 antibody - 344 004

GAP43 is involved in axonal differentiation

Guinea pig polyclonal antiserum

Cat. No.: 344 004

Amount: 100 µl

Price: $365.00

Cat. No. 344 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 226 from rat GAP43 (UniProt Id: P07936)
Reactivity	Reacts with: rat (P07936), mouse (P06837). Other species not tested yet.
Data sheet	344_004.pdf

Cat. No.: 344 004

Amount: 100 µl

Price: $365.00

ICC

IHC

Background

The neuronal growth-associated protein GAP 43 plays an important role in nerve growth and is a major component of the motile "growth cones" that form the tips of elongating axons. It is the major substrate for protein kinase C and is associated with axonal and dendritic filopodia induction. GAP 43 is also known as Neuromodulin, B-50, P-57, pp46, and F1.

1 ) Gnanapavan S et al., J. Neuroimmunol. (2014)
2 ) Donnelly CJ et al., J. Neurosci. (2013)
3 ) Grasselli G et al., PLoS ONE (2011)
4 ) Spencer SA et al., J. Biol. Chem. (1992)
5 ) Rosenthal A et al., EMBO J. (1987)

Protocols

Standard protocol

ICC

IHC

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