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GABA-A receptor gamma2 antibody extracellular - 224 004

GABA-A receptors are involved in inhibitory neurotransmission
Guinea pig polyclonal antiserum
Cat. No.: 224 004
Amount: 100 µl
Price: $370.00
Cat. No. 224 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: yes limited (see remarks)
IP: yes
ICC: 1 : 500 (see remarks) gallery  
IHC: 1 : 500 up to 1 : 2000 (see remarks) gallery  
IHC-P: not tested yet
IHC-Fr: 1 : 500 (see remarks) gallery  
Immunogen Synthetic peptide corresponding to AA 39 to 67 from mouse GABA-A receptor γ2 (UniProt Id: P22723)
Reactivity Reacts with: human (P18507), rat (P18508), mouse (P22723).
Other species not tested yet.
Specificity Specific for GABA-A receptor γ2. Does not discriminate between the L and S form.
Matching control protein/peptide 224-0P
Remarks

WB: The antibody is less sensitive in westernblotting compared to the rabbit antibody. GABA-A receptor gamma2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ICC: This antibody is also suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.
IHC: For best results use the protocol of Schneider Gasser et al., 2006.

Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical staining.
IHC-Fr: PFA fixation is recommended.
Data sheet 224_004.pdf

References for GABA-A receptor gamma2 - 224 004

Artesunate restores the levels of inhibitory synapse proteins and reduces amyloid-β and C-terminal fragments (CTFs) of the amyloid precursor protein in an AD-mouse model.
Kiss E, Kins S, Zöller Y, Schilling S, Gorgas K, Groß D, Schlicksupp A, Rosner R, Kirsch J, Kuhse J
Molecular and cellular neurosciences (2021) 113: 103624. 224 004 WB, IHC; tested species: mouse
Estradiol modulates the efficacy of synaptic inhibition by decreasing the dwell time of GABAA receptors at inhibitory synapses.
Mukherjee J, Cardarelli RA, Cantaut-Belarif Y, Deeb TZ, Srivastava DP, Tyagarajan SK, Pangalos MN, Triller A, Maguire J, Brandon NJ, Moss SJ, et al.
Proceedings of the National Academy of Sciences of the United States of America (2017) 11444: 11763-11768. 224 004 ICC, WB
The kinesin KIF21B participates in the cell surface delivery of γ2 subunit-containing GABAA receptors.
Labonté D, Thies E, Kneussel M
European journal of cell biology () 938-9: 338-46. 224 004 IP, ICC
Structural and functional reorganization of inhibitory synapses by activity-dependent cleavage of neuroligin-2.
Xu N, Cao R, Chen SY, Gou XZ, Wang B, Luo HM, Gao F, Tang AH
Proceedings of the National Academy of Sciences of the United States of America (2024) 12118: e2314541121. 224 004 ICC; tested species: mouse
Distinct mechanisms drive sequential internalization and degradation of GABAARs during global ischemia and reperfusion injury.
Garcia JD, Wolfe SE, Stewart AR, Tiemeier E, Gookin SE, Guerrero MB, Quillinan N, Smith KR
iScience (2023) 2610: 108061. 224 004 ICC, UPTAKE; tested species: rat
Acute reorganization of postsynaptic GABAA receptors reveals the functional impact of molecular nanoarchitecture at inhibitory synapses.
Olah SS, Kareemo DJ, Buchta WC, Sinnen BL, Miller CN, Actor-Engel HS, Gookin SE, Winborn CS, Kleinjan MS, Crosby KC, Aoto J, et al.
Cell reports (2023) 4211: 113331. 224 004 ICC; tested species: rat
AMPA and GABAA receptor nanodomains assemble in the absence of synaptic neurotransmitter release.
Ramsay HJ, Gookin SE, Ramsey AM, Kareemo DJ, Crosby KC, Stich DG, Olah SS, Actor-Engel HS, Smith KR, Kennedy MJ
Frontiers in molecular neuroscience (2023) 16: 1232795. 224 004 ICC; tested species: rat
Phosphorylation of neuroligin-2 by PKA regulates its cell surface abundance and synaptic stabilization.
Halff EF, Hannan S, Kwanthongdee J, Lesept F, Smart TG, Kittler JT
Science signaling (2022) 15739: eabg2505. 224 004 ICC; tested species: rat
Conformational state-dependent regulation of GABAA receptor diffusion and subsynaptic domains.
Merlaud Z, Marques X, Russeau M, Saade U, Tostain M, Moutkine I, Gielen M, Corringer PJ, Lévi S
iScience (2022) 2511: 105467. 224 004 ICC; tested species: rat
Dually innervated dendritic spines develop in the absence of excitatory activity and resist plasticity through tonic inhibitory crosstalk.
Kleinjan MS, Buchta WC, Ogelman R, Hwang IW, Kuwajima M, Hubbard DD, Kareemo DJ, Prikhodko O, Olah SL, Gomez Wulschner LE, Abraham WC, et al.
Neuron (2022) : . 224 004 ICC; tested species: mouse
Biallelic gephyrin variants lead to impaired GABAergic inhibition in a patient with developmental and epileptic encephalopathy.
Macha A, Liebsch F, Fricke S, Hetsch F, Neuser F, Johannes L, Kress V, Djémié T, Santamaria-Araujo JA, Vilain C, Aeby A, et al.
Human molecular genetics (2022) 316: 901-913. 224 004 ICC; tested species: mouse
Stepwise disassembly of GABAergic synapses during pathogenic excitotoxicity.
Garcia JD, Gookin SE, Crosby KC, Schwartz SL, Tiemeier E, Kennedy MJ, Dell'Acqua ML, Herson PS, Quillinan N, Smith KR
Cell reports (2021) 3712: 110142. 224 004 ICC; tested species: rat
Artemisinin-treatment in pre-symptomatic APP-PS1 mice increases gephyrin phosphorylation at Ser270: a modification regulating postsynaptic GABAAR density.
Kiss E, Kins S, Gorgas K, Orlik M, Fischer C, Endres K, Schlicksupp A, Kirsch J, Kuhse J
Biological chemistry (2021) : . 224 004 ICC, IHC; tested species: mouse,rat
Local miRNA-Dependent Translational Control of GABAAR Synthesis during Inhibitory Long-Term Potentiation.
Rajgor D, Purkey AM, Sanderson JL, Welle TM, Garcia JD, Dell'Acqua ML, Smith KR
Cell reports (2020) 3112: 107785. 224 004 ICC; tested species: rat
Nanoscale Subsynaptic Domains Underlie the Organization of the Inhibitory Synapse.
Crosby KC, Gookin SE, Garcia JD, Hahm KM, Dell'Acqua ML, Smith KR
Cell reports (2019) 2612: 3284-3297.e3. 224 004 ICC, IHC; tested species: rat
Autism and Schizophrenia-Associated CYFIP1 Regulates the Balance of Synaptic Excitation and Inhibition.
Davenport EC, Szulc BR, Drew J, Taylor J, Morgan T, Higgs NF, López-Doménech G, Kittler JT
Cell reports (2019) 268: 2037-2051.e6. 224 004 ICC; tested species: mouse
Cell surface expression of homomeric GABAA receptors depends on single residues in subunit transmembrane domains.
Hannan S, Smart TG
The Journal of biological chemistry (2018) : . 224 004 ICC; tested species: human
Estradiol modulates the efficacy of synaptic inhibition by decreasing the dwell time of GABAA receptors at inhibitory synapses.
Mukherjee J, Cardarelli RA, Cantaut-Belarif Y, Deeb TZ, Srivastava DP, Tyagarajan SK, Pangalos MN, Triller A, Maguire J, Brandon NJ, Moss SJ, et al.
Proceedings of the National Academy of Sciences of the United States of America (2017) 11444: 11763-11768. 224 004 ICC, WB
Effects of distinct collybistin isoforms on the formation of GABAergic synapses in hippocampal neurons.
Körber C, Richter A, Kaiser M, Schlicksupp A, Mükusch S, Kuner T, Kirsch J, Kuhse J
Molecular and cellular neurosciences (2012) 503-4: 250-9. 224 004 ICC
The kinesin KIF21B participates in the cell surface delivery of γ2 subunit-containing GABAA receptors.
Labonté D, Thies E, Kneussel M
European journal of cell biology () 938-9: 338-46. 224 004 IP, ICC
Increased body weight in mice with fragile X messenger ribonucleoprotein 1 (Fmr1) gene mutation is associated with hypothalamic dysfunction.
Ruggiero-Ruff RE, Villa PA, Hijleh SA, Avalos B, DiPatrizio NV, Haga-Yamanaka S, Coss D
Scientific reports (2023) 131: 12666. 224 004 IHC; tested species: mouse
Different priming states of synaptic vesicles underlie distinct release probabilities at hippocampal excitatory synapses.
Aldahabi M, Balint F, Holderith N, Lorincz A, Reva M, Nusser Z
Neuron (2022) : . 224 004 IHC; tested species: mouse
Vision-dependent specification of cell types and function in the developing cortex.
Cheng S, Butrus S, Tan L, Xu R, Sagireddy S, Trachtenberg JT, Shekhar K, Zipursky SL
Cell (2022) 1852: 311-327.e24. 224 004 IHC; tested species: mouse
Artesunate restores the levels of inhibitory synapse proteins and reduces amyloid-β and C-terminal fragments (CTFs) of the amyloid precursor protein in an AD-mouse model.
Kiss E, Kins S, Zöller Y, Schilling S, Gorgas K, Groß D, Schlicksupp A, Rosner R, Kirsch J, Kuhse J
Molecular and cellular neurosciences (2021) 113: 103624. 224 004 WB, IHC; tested species: mouse
Artemisinin-treatment in pre-symptomatic APP-PS1 mice increases gephyrin phosphorylation at Ser270: a modification regulating postsynaptic GABAAR density.
Kiss E, Kins S, Gorgas K, Orlik M, Fischer C, Endres K, Schlicksupp A, Kirsch J, Kuhse J
Biological chemistry (2021) : . 224 004 ICC, IHC; tested species: mouse,rat
Nanoscale Subsynaptic Domains Underlie the Organization of the Inhibitory Synapse.
Crosby KC, Gookin SE, Garcia JD, Hahm KM, Dell'Acqua ML, Smith KR
Cell reports (2019) 2612: 3284-3297.e3. 224 004 ICC, IHC; tested species: rat
Disinhibition of somatostatin-positive GABAergic interneurons results in an anxiolytic and antidepressant-like brain state.
Fuchs T, Jefferson SJ, Hooper A, Yee PH, Maguire J, Luscher B
Molecular psychiatry (2017) 226: 920-930. 224 004 IHC
Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 224 004 IHC
Forebrain-specific loss of synaptic GABAA receptors results in altered neuronal excitability and synaptic plasticity in mice.
O'Sullivan GA, Jedlicka P, Chen HX, Kalbouneh H, Ippolito A, Deller T, Nawrotzki RA, Kuhse J, Kalaidzidis YL, Kirsch J, Schwarzacher SW, et al.
Molecular and cellular neurosciences (2016) 72: 101-13. 224 004 IHC; tested species: mouse
Distinct mechanisms drive sequential internalization and degradation of GABAARs during global ischemia and reperfusion injury.
Garcia JD, Wolfe SE, Stewart AR, Tiemeier E, Gookin SE, Guerrero MB, Quillinan N, Smith KR
iScience (2023) 2610: 108061. 224 004 ICC, UPTAKE; tested species: rat
Nano-organization of spontaneous GABAergic transmission directs its autonomous function in neuronal signaling.
Guzikowski NJ, Kavalali ET
Cell reports (2022) 406: 111172. 224 004 UPTAKE; tested species: rat
SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling.
Halff EF, Szulc BR, Lesept F, Kittler JT
Cell reports (2019) 299: 2599-2607.e6. 224 004 UPTAKE; tested species: rat
Cat. No.: 224 004
Amount: 100 µl
Price: $370.00
Artesunate restores the levels of inhibitory synapse proteins and reduces amyloid-β and C-terminal fragments (CTFs) of the amyloid precursor protein in an AD-mouse model.
Kiss E, Kins S, Zöller Y, Schilling S, Gorgas K, Groß D, Schlicksupp A, Rosner R, Kirsch J, Kuhse J
Molecular and cellular neurosciences (2021) 113: 103624. 224 004 WB, IHC; tested species: mouse
Estradiol modulates the efficacy of synaptic inhibition by decreasing the dwell time of GABAA receptors at inhibitory synapses.
Mukherjee J, Cardarelli RA, Cantaut-Belarif Y, Deeb TZ, Srivastava DP, Tyagarajan SK, Pangalos MN, Triller A, Maguire J, Brandon NJ, Moss SJ, et al.
Proceedings of the National Academy of Sciences of the United States of America (2017) 11444: 11763-11768. 224 004 ICC, WB
The kinesin KIF21B participates in the cell surface delivery of γ2 subunit-containing GABAA receptors.
Labonté D, Thies E, Kneussel M
European journal of cell biology () 938-9: 338-46. 224 004 IP, ICC
Structural and functional reorganization of inhibitory synapses by activity-dependent cleavage of neuroligin-2.
Xu N, Cao R, Chen SY, Gou XZ, Wang B, Luo HM, Gao F, Tang AH
Proceedings of the National Academy of Sciences of the United States of America (2024) 12118: e2314541121. 224 004 ICC; tested species: mouse
Distinct mechanisms drive sequential internalization and degradation of GABAARs during global ischemia and reperfusion injury.
Garcia JD, Wolfe SE, Stewart AR, Tiemeier E, Gookin SE, Guerrero MB, Quillinan N, Smith KR
iScience (2023) 2610: 108061. 224 004 ICC, UPTAKE; tested species: rat
Acute reorganization of postsynaptic GABAA receptors reveals the functional impact of molecular nanoarchitecture at inhibitory synapses.
Olah SS, Kareemo DJ, Buchta WC, Sinnen BL, Miller CN, Actor-Engel HS, Gookin SE, Winborn CS, Kleinjan MS, Crosby KC, Aoto J, et al.
Cell reports (2023) 4211: 113331. 224 004 ICC; tested species: rat
AMPA and GABAA receptor nanodomains assemble in the absence of synaptic neurotransmitter release.
Ramsay HJ, Gookin SE, Ramsey AM, Kareemo DJ, Crosby KC, Stich DG, Olah SS, Actor-Engel HS, Smith KR, Kennedy MJ
Frontiers in molecular neuroscience (2023) 16: 1232795. 224 004 ICC; tested species: rat
Phosphorylation of neuroligin-2 by PKA regulates its cell surface abundance and synaptic stabilization.
Halff EF, Hannan S, Kwanthongdee J, Lesept F, Smart TG, Kittler JT
Science signaling (2022) 15739: eabg2505. 224 004 ICC; tested species: rat
Conformational state-dependent regulation of GABAA receptor diffusion and subsynaptic domains.
Merlaud Z, Marques X, Russeau M, Saade U, Tostain M, Moutkine I, Gielen M, Corringer PJ, Lévi S
iScience (2022) 2511: 105467. 224 004 ICC; tested species: rat
Dually innervated dendritic spines develop in the absence of excitatory activity and resist plasticity through tonic inhibitory crosstalk.
Kleinjan MS, Buchta WC, Ogelman R, Hwang IW, Kuwajima M, Hubbard DD, Kareemo DJ, Prikhodko O, Olah SL, Gomez Wulschner LE, Abraham WC, et al.
Neuron (2022) : . 224 004 ICC; tested species: mouse
Biallelic gephyrin variants lead to impaired GABAergic inhibition in a patient with developmental and epileptic encephalopathy.
Macha A, Liebsch F, Fricke S, Hetsch F, Neuser F, Johannes L, Kress V, Djémié T, Santamaria-Araujo JA, Vilain C, Aeby A, et al.
Human molecular genetics (2022) 316: 901-913. 224 004 ICC; tested species: mouse
Stepwise disassembly of GABAergic synapses during pathogenic excitotoxicity.
Garcia JD, Gookin SE, Crosby KC, Schwartz SL, Tiemeier E, Kennedy MJ, Dell'Acqua ML, Herson PS, Quillinan N, Smith KR
Cell reports (2021) 3712: 110142. 224 004 ICC; tested species: rat
Artemisinin-treatment in pre-symptomatic APP-PS1 mice increases gephyrin phosphorylation at Ser270: a modification regulating postsynaptic GABAAR density.
Kiss E, Kins S, Gorgas K, Orlik M, Fischer C, Endres K, Schlicksupp A, Kirsch J, Kuhse J
Biological chemistry (2021) : . 224 004 ICC, IHC; tested species: mouse,rat
Local miRNA-Dependent Translational Control of GABAAR Synthesis during Inhibitory Long-Term Potentiation.
Rajgor D, Purkey AM, Sanderson JL, Welle TM, Garcia JD, Dell'Acqua ML, Smith KR
Cell reports (2020) 3112: 107785. 224 004 ICC; tested species: rat
Nanoscale Subsynaptic Domains Underlie the Organization of the Inhibitory Synapse.
Crosby KC, Gookin SE, Garcia JD, Hahm KM, Dell'Acqua ML, Smith KR
Cell reports (2019) 2612: 3284-3297.e3. 224 004 ICC, IHC; tested species: rat
Autism and Schizophrenia-Associated CYFIP1 Regulates the Balance of Synaptic Excitation and Inhibition.
Davenport EC, Szulc BR, Drew J, Taylor J, Morgan T, Higgs NF, López-Doménech G, Kittler JT
Cell reports (2019) 268: 2037-2051.e6. 224 004 ICC; tested species: mouse
Cell surface expression of homomeric GABAA receptors depends on single residues in subunit transmembrane domains.
Hannan S, Smart TG
The Journal of biological chemistry (2018) : . 224 004 ICC; tested species: human
Estradiol modulates the efficacy of synaptic inhibition by decreasing the dwell time of GABAA receptors at inhibitory synapses.
Mukherjee J, Cardarelli RA, Cantaut-Belarif Y, Deeb TZ, Srivastava DP, Tyagarajan SK, Pangalos MN, Triller A, Maguire J, Brandon NJ, Moss SJ, et al.
Proceedings of the National Academy of Sciences of the United States of America (2017) 11444: 11763-11768. 224 004 ICC, WB
Effects of distinct collybistin isoforms on the formation of GABAergic synapses in hippocampal neurons.
Körber C, Richter A, Kaiser M, Schlicksupp A, Mükusch S, Kuner T, Kirsch J, Kuhse J
Molecular and cellular neurosciences (2012) 503-4: 250-9. 224 004 ICC
The kinesin KIF21B participates in the cell surface delivery of γ2 subunit-containing GABAA receptors.
Labonté D, Thies E, Kneussel M
European journal of cell biology () 938-9: 338-46. 224 004 IP, ICC
Increased body weight in mice with fragile X messenger ribonucleoprotein 1 (Fmr1) gene mutation is associated with hypothalamic dysfunction.
Ruggiero-Ruff RE, Villa PA, Hijleh SA, Avalos B, DiPatrizio NV, Haga-Yamanaka S, Coss D
Scientific reports (2023) 131: 12666. 224 004 IHC; tested species: mouse
Different priming states of synaptic vesicles underlie distinct release probabilities at hippocampal excitatory synapses.
Aldahabi M, Balint F, Holderith N, Lorincz A, Reva M, Nusser Z
Neuron (2022) : . 224 004 IHC; tested species: mouse
Vision-dependent specification of cell types and function in the developing cortex.
Cheng S, Butrus S, Tan L, Xu R, Sagireddy S, Trachtenberg JT, Shekhar K, Zipursky SL
Cell (2022) 1852: 311-327.e24. 224 004 IHC; tested species: mouse
Artesunate restores the levels of inhibitory synapse proteins and reduces amyloid-β and C-terminal fragments (CTFs) of the amyloid precursor protein in an AD-mouse model.
Kiss E, Kins S, Zöller Y, Schilling S, Gorgas K, Groß D, Schlicksupp A, Rosner R, Kirsch J, Kuhse J
Molecular and cellular neurosciences (2021) 113: 103624. 224 004 WB, IHC; tested species: mouse
Artemisinin-treatment in pre-symptomatic APP-PS1 mice increases gephyrin phosphorylation at Ser270: a modification regulating postsynaptic GABAAR density.
Kiss E, Kins S, Gorgas K, Orlik M, Fischer C, Endres K, Schlicksupp A, Kirsch J, Kuhse J
Biological chemistry (2021) : . 224 004 ICC, IHC; tested species: mouse,rat
Nanoscale Subsynaptic Domains Underlie the Organization of the Inhibitory Synapse.
Crosby KC, Gookin SE, Garcia JD, Hahm KM, Dell'Acqua ML, Smith KR
Cell reports (2019) 2612: 3284-3297.e3. 224 004 ICC, IHC; tested species: rat
Disinhibition of somatostatin-positive GABAergic interneurons results in an anxiolytic and antidepressant-like brain state.
Fuchs T, Jefferson SJ, Hooper A, Yee PH, Maguire J, Luscher B
Molecular psychiatry (2017) 226: 920-930. 224 004 IHC
Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 224 004 IHC
Forebrain-specific loss of synaptic GABAA receptors results in altered neuronal excitability and synaptic plasticity in mice.
O'Sullivan GA, Jedlicka P, Chen HX, Kalbouneh H, Ippolito A, Deller T, Nawrotzki RA, Kuhse J, Kalaidzidis YL, Kirsch J, Schwarzacher SW, et al.
Molecular and cellular neurosciences (2016) 72: 101-13. 224 004 IHC; tested species: mouse
Distinct mechanisms drive sequential internalization and degradation of GABAARs during global ischemia and reperfusion injury.
Garcia JD, Wolfe SE, Stewart AR, Tiemeier E, Gookin SE, Guerrero MB, Quillinan N, Smith KR
iScience (2023) 2610: 108061. 224 004 ICC, UPTAKE; tested species: rat
Nano-organization of spontaneous GABAergic transmission directs its autonomous function in neuronal signaling.
Guzikowski NJ, Kavalali ET
Cell reports (2022) 406: 111172. 224 004 UPTAKE; tested species: rat
SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling.
Halff EF, Szulc BR, Lesept F, Kittler JT
Cell reports (2019) 299: 2599-2607.e6. 224 004 UPTAKE; tested species: rat
ICC
ICC
IHC
IHC
IHC
IHC-Fr
IHC-Fr
IHC-Fr
Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.
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