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Home > Products > 224 411BT

GABA-A receptor beta3 antibody - 224 411BT

GABA-A receptors are involved in inhibitory neurotransmission
Mouse monoclonal purified IgG
Cat. No.: 224 411BT
Amount: 100 µg
Price: $465.00
Cat. No. 224 411BT 100 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

 For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 1000 (AP staining) (see remarks)
IP: yes
ICC: 1 : 500 gallery  
IHC: not tested yet (see remarks)
IHC-P: not tested yet
Label biotin
Clone 261F6
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 390 to 429 from rat GABA-A receptor β3 (UniProt Id: P63079)
Reactivity Reacts with: rat (P63079), mouse (P63080).
Other species not tested yet.
Remarks

WB: Less sensitive compared to the polyclonal antibodies (224 403 and 224 404).
IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).
Data sheet 224_411bt.pdf
Cat. No.: 224 411BT
Amount: 100 µg
Price: $465.00
ICC
Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.
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