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GABA-A receptor beta3 antibody - 224 404

GABA-A receptors are involved in inhibitory neurotransmission
Guinea pig polyclonal antiserum
Cat. No.: 224 404
Amount: 100 µl
Price: $365.00
Cat. No. 224 404 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not tested yet
EM: yes
Immunogen Recombinant protein corresponding to AA 390 to 429 from rat GABA-A receptor β3 (UniProt Id: P63079)
Reactivity Reacts with: rat (P63079), mouse (P63080).
Other species not tested yet.
Remarks

WB:  GABA-A receptor beta3 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).

Data sheet 224_404.pdf

References for GABA-A receptor beta3 - 224 404

Synaptic GABAA receptor clustering without the γ2 subunit.
Kerti-Szigeti K, Nusser Z, Eyre MD
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3431: 10219-33. 224 404 IHC
Cat. No.: 224 404
Amount: 100 µl
Price: $365.00
Synaptic GABAA receptor clustering without the γ2 subunit.
Kerti-Szigeti K, Nusser Z, Eyre MD
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3431: 10219-33. 224 404 IHC
Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.