Synaptic Systems - GABA-A receptor beta3

Cat. No.: 224 404

Amount: 100 µl

Price: $370.00

Cat. No. 224 404	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 (see remarks) gallery IHC-P: not tested yet EM: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen	Recombinant protein corresponding to AA 390 to 429 from rat GABA-A receptor β3 (UniProt Id: P63079)
Reactivity	Reacts with: rat (P63079), mouse (P63080). Other species not tested yet.
Remarks	WB: GABA-A receptor beta3 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).
Data sheet	224_404.pdf

References for GABA-A receptor beta3 - 224 404

The TMEM132B-GABAA receptor complex controls alcohol actions in the brain.
Wang G, Peng S, Reyes Mendez M, Keramidas A, Castellano D, Wu K, Han W, Tian Q, Dong L, Li Y, Lu W, et al.
Cell (2024) 18723: 6649-6668.e35. 224 404 ICC; tested species: mouse

Synaptic GABAA receptor clustering without the γ2 subunit.
Kerti-Szigeti K, Nusser Z, Eyre MD
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3431: 10219-33. 224 404 IHC

Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 224 404 EM

Cat. No.: 224 404

Amount: 100 µl

Price: $370.00

Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 224 404 EM

ICC

IHC

Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.

1 ) Sassoè-Pognetto M et al., Ann. N. Y. Acad. Sci. (2009)
2 ) Viltono L et al., J. Comp. Neurol. (2008)
3 ) Kralic JE et al., J. Comp. Neurol. (2006)
4 ) Essrich C et al., Nat. Neurosci. (1998)
5 ) Fritschy JM et al., J. Comp. Neurol. (1995)
6 ) Benke D et al., J. Biol. Chem. (1994)
7 ) Fritschy JM et al., Proc. Natl. Acad. Sci. U.S.A. (1992)

Protocols

Standard protocol

ICC

IHC

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GABA-A receptor beta3 antibody - 224 404

References for GABA-A receptor beta3 - 224 404