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GABA-A receptor beta3 antibody - 224 403

GABA-A receptors are involved in inhibitory neurotransmission
Rabbit polyclonal purified antibody
Cat. No.: 224 403
Amount: 50 µg
Price: $375.00
Cat. No. 224 403 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 390 to 429 from rat GABA-A receptor β3 (UniProt Id: P63079)
Reactivity Reacts with: rat (P63079), mouse (P63080).
Other species not tested yet.
Remarks

WB:  GABA-A receptor beta3 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).

Data sheet 224_403.pdf

References for GABA-A receptor beta3 - 224 403

Essential role for InSyn1 in dystroglycan complex integrity and cognitive behaviors in mice.
Uezu A, Hisey E, Kobayashi Y, Gao Y, Bradshaw TW, Devlin P, Rodriguiz R, Tata PR, Soderling S
eLife (2019) 8: . 224 403 ICC; tested species: mouse
Transient expression of a GABA receptor subunit during early development is critical for inhibitory synapse maturation and function.
Sinha R, Grimes WN, Wallin J, Ebbinghaus BN, Luu K, Cherry T, Rieke F, Rudolph U, Wong RO, Hoon M
Current biology : CB (2021) 3119: 4314-4326.e5. 224 403 IHC; tested species: mouse
Encephalitis patient-derived monoclonal GABAA receptor antibodies cause epileptic seizures.
Kreye J, Wright SK, van Casteren A, Stöffler L, Machule ML, Reincke SM, Nikolaus M, van Hoof S, Sanchez-Sendin E, Homeyer MA, Cordero Gómez C, et al.
The Journal of experimental medicine (2021) 21811: . 224 403 IHC; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 224 403 IHC; tested species: rat
Cat. No.: 224 403
Amount: 50 µg
Price: $375.00
Essential role for InSyn1 in dystroglycan complex integrity and cognitive behaviors in mice.
Uezu A, Hisey E, Kobayashi Y, Gao Y, Bradshaw TW, Devlin P, Rodriguiz R, Tata PR, Soderling S
eLife (2019) 8: . 224 403 ICC; tested species: mouse
Transient expression of a GABA receptor subunit during early development is critical for inhibitory synapse maturation and function.
Sinha R, Grimes WN, Wallin J, Ebbinghaus BN, Luu K, Cherry T, Rieke F, Rudolph U, Wong RO, Hoon M
Current biology : CB (2021) 3119: 4314-4326.e5. 224 403 IHC; tested species: mouse
Encephalitis patient-derived monoclonal GABAA receptor antibodies cause epileptic seizures.
Kreye J, Wright SK, van Casteren A, Stöffler L, Machule ML, Reincke SM, Nikolaus M, van Hoof S, Sanchez-Sendin E, Homeyer MA, Cordero Gómez C, et al.
The Journal of experimental medicine (2021) 21811: . 224 403 IHC; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 224 403 IHC; tested species: rat
Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.