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GABA-A receptor alpha3 antibody extracellular - 224 303 K.O.

GABA-A receptors are involved in inhibitory neurotransmission
Rabbit polyclonal purified antibody
Cat. No.: 224 303
Amount: 50 µg
Price: $455.00
Cat. No. 224 303 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not tested yet
Immunogen Synthetic peptide corresponding to AA 29 to 43 from rat GABA-A receptor α3 (UniProt Id: P20236)
Reactivity Reacts with: rat (P20236), mouse (P26049).
Other species not tested yet.
Specificity K.O. validated PubMed: 33119191
Matching control protein/peptide 224-3P
Remarks

WB:  GABA-A receptor alpha3 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).
Data sheet 224_303.pdf

References for GABA-A receptor alpha3 - 224 303

Deletion of histamine H2 receptor in VTA dopaminergic neurons of mice induces behavior reminiscent of mania.
Ma S, Ma Q, Hu S, Mo X, Zhu C, Zhang X, Jia Z, Tang L, Jiang L, Cui Y, Chen Z, et al.
Cell reports (2024) 439: 114717. 224 303 WB; tested species: mouse
Encephalitis patient-derived monoclonal GABAA receptor antibodies cause epileptic seizures.
Kreye J, Wright SK, van Casteren A, Stöffler L, Machule ML, Reincke SM, Nikolaus M, van Hoof S, Sanchez-Sendin E, Homeyer MA, Cordero Gómez C, et al.
The Journal of experimental medicine (2021) 21811: . 224 303 ICC; tested species: rat
The α3 subunit of GABAA receptors promotes formation of inhibitory synapses in the absence of collybistin.
Wagner S, Lee C, Rojas L, Specht CG, Rhee J, Brose N, Papadopoulos T
The Journal of biological chemistry () 296: 100709. 224 303 ICC, IHC; tested species: mouse
Cytoplasmic FUS triggers early behavioral alterations linked to cortical neuronal hyperactivity and inhibitory synaptic defects.
Scekic-Zahirovic J, Sanjuan-Ruiz I, Kan V, Megat S, De Rossi P, Dieterlé S, Cassel R, Jamet M, Kessler P, Wiesner D, Tzeplaeff L, et al.
Nature communications (2021) 121: 3028. 224 303 IHC; tested species: mouse
Identification of intraneuronal amyloid beta oligomers in locus coeruleus neurons of Alzheimer's patients and their potential impact on inhibitory neurotransmitter receptors and neuronal excitability.
Kelly L, Seifi M, Ma R, Mitchell SJ, Rudolph U, Viola KL, Klein WL, Lambert JJ, Swinny JD
Neuropathology and applied neurobiology (2021) 474: 488-505. 224 303 IHC; KO verified; tested species: mouse
Enhancing neuronal chloride extrusion rescues α2/α3 GABAA-mediated analgesia in neuropathic pain.
Lorenzo LE, Godin AG, Ferrini F, Bachand K, Plasencia-Fernandez I, Labrecque S, Girard AA, Boudreau D, Kianicka I, Gagnon M, Doyon N, et al.
Nature communications (2020) 111: 869. 224 303 IHC; tested species: rat
Spatiotemporal Distribution of GABAA Receptor Subunits Within Layer II of Mouse Medial Entorhinal Cortex: Implications for Grid Cell Excitability.
Berggaard N, Seifi M, van der Want JJL, Swinny JD
Frontiers in neuroanatomy (2018) 12: 46. 224 303 IHC; tested species: mouse
Long-term behavioral programming induced by peripuberty stress in rats is accompanied by GABAergic-related alterations in the Amygdala.
Tzanoulinou S, García-Mompó C, Castillo-Gómez E, Veenit V, Nacher J, Sandi C
PloS one (2014) 94: e94666. 224 303 IHC; tested species: rat
Only a Minority of the Inhibitory Inputs to Cerebellar Golgi Cells Originates from Local GABAergic Cells.
Eyre MD, Nusser Z
eNeuro () 32: . 224 303 IHC
The α3 subunit of GABAA receptors promotes formation of inhibitory synapses in the absence of collybistin.
Wagner S, Lee C, Rojas L, Specht CG, Rhee J, Brose N, Papadopoulos T
The Journal of biological chemistry () 296: 100709. 224 303 ICC, IHC; tested species: mouse
Cat. No.: 224 303
Amount: 50 µg
Price: $455.00
Deletion of histamine H2 receptor in VTA dopaminergic neurons of mice induces behavior reminiscent of mania.
Ma S, Ma Q, Hu S, Mo X, Zhu C, Zhang X, Jia Z, Tang L, Jiang L, Cui Y, Chen Z, et al.
Cell reports (2024) 439: 114717. 224 303 WB; tested species: mouse
Encephalitis patient-derived monoclonal GABAA receptor antibodies cause epileptic seizures.
Kreye J, Wright SK, van Casteren A, Stöffler L, Machule ML, Reincke SM, Nikolaus M, van Hoof S, Sanchez-Sendin E, Homeyer MA, Cordero Gómez C, et al.
The Journal of experimental medicine (2021) 21811: . 224 303 ICC; tested species: rat
The α3 subunit of GABAA receptors promotes formation of inhibitory synapses in the absence of collybistin.
Wagner S, Lee C, Rojas L, Specht CG, Rhee J, Brose N, Papadopoulos T
The Journal of biological chemistry () 296: 100709. 224 303 ICC, IHC; tested species: mouse
Cytoplasmic FUS triggers early behavioral alterations linked to cortical neuronal hyperactivity and inhibitory synaptic defects.
Scekic-Zahirovic J, Sanjuan-Ruiz I, Kan V, Megat S, De Rossi P, Dieterlé S, Cassel R, Jamet M, Kessler P, Wiesner D, Tzeplaeff L, et al.
Nature communications (2021) 121: 3028. 224 303 IHC; tested species: mouse
Identification of intraneuronal amyloid beta oligomers in locus coeruleus neurons of Alzheimer's patients and their potential impact on inhibitory neurotransmitter receptors and neuronal excitability.
Kelly L, Seifi M, Ma R, Mitchell SJ, Rudolph U, Viola KL, Klein WL, Lambert JJ, Swinny JD
Neuropathology and applied neurobiology (2021) 474: 488-505. 224 303 IHC; KO verified; tested species: mouse
Enhancing neuronal chloride extrusion rescues α2/α3 GABAA-mediated analgesia in neuropathic pain.
Lorenzo LE, Godin AG, Ferrini F, Bachand K, Plasencia-Fernandez I, Labrecque S, Girard AA, Boudreau D, Kianicka I, Gagnon M, Doyon N, et al.
Nature communications (2020) 111: 869. 224 303 IHC; tested species: rat
Spatiotemporal Distribution of GABAA Receptor Subunits Within Layer II of Mouse Medial Entorhinal Cortex: Implications for Grid Cell Excitability.
Berggaard N, Seifi M, van der Want JJL, Swinny JD
Frontiers in neuroanatomy (2018) 12: 46. 224 303 IHC; tested species: mouse
Long-term behavioral programming induced by peripuberty stress in rats is accompanied by GABAergic-related alterations in the Amygdala.
Tzanoulinou S, García-Mompó C, Castillo-Gómez E, Veenit V, Nacher J, Sandi C
PloS one (2014) 94: e94666. 224 303 IHC; tested species: rat
Only a Minority of the Inhibitory Inputs to Cerebellar Golgi Cells Originates from Local GABAergic Cells.
Eyre MD, Nusser Z
eNeuro () 32: . 224 303 IHC
The α3 subunit of GABAA receptors promotes formation of inhibitory synapses in the absence of collybistin.
Wagner S, Lee C, Rojas L, Specht CG, Rhee J, Brose N, Papadopoulos T
The Journal of biological chemistry () 296: 100709. 224 303 ICC, IHC; tested species: mouse
WB
ICC
IHC
Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.
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