gamma-Enolase antibody - 230 004

gamma or neural Enolase is a neuron-specific enolase subunit

Guinea pig polyclonal antiserum

Cat. No.: 230 004

Amount: 100 µl

Price: $365.00

Cat. No. 230 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 418 to 434 from rat γ-Enolase (UniProt Id: P07323)
Reactivity	Reacts with: rat (P07323), mouse (P17183). Other species not tested yet.
Specificity	Specific for γ-enolase.
Matching control protein/peptide	230-0P
Data sheet	230_004.pdf

Cat. No.: 230 004

Amount: 100 µl

Price: $365.00

ICC

IHC

Background

The glycolytic enzyme enolase (2-phospho-d-glycerate hydrolyase; EC 4.2.1.11) occurs as heterodimers composed of three independently encoded isoforms (α, β, γ). Most tissues express the ubiquitous αα dimer. In the nervous system it is replaced by αγ,γγ, and in the striated muscle by αβ, ββ.

1 ) Diao WF et al., Neuroscience (2008)
2 ) Merkulova T et al., Eur. J. Biochem. (2000)
3 ) Merkulova T et al., Biochem. J. (1997)

Protocols

Standard protocol

ICC

IHC

OTHER

Preabsorbtion

Other products for this target