Cat. No.: 702-rbCpH
Amount: 50 µg
Price:
$450.00
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pH-sensitive probe fluorescent only under acidic conditions
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| Cat. No. 702-rbCpH |
50 µg affinity purified Fab fragments, lyophilized. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light to minimize photo bleeching of the fluorescent dye. |
| Applications | |
| Label | CypHer5E |
| Reactivity |
Reacts with: rabbit. Other species not tested yet. |
| Remarks |
Antigen-purified primary antibodies are recommended. Unpurified rabbit serum or total IgG preparations may contain considerable amounts of unspecific IgGs that will bind plenty of the rbFab-CpH resulting in a lower overall labelling efficiency. The pH sensitive dye only regaines its fluorescence after the acidification of the targeted cellular compartment. |
| Data sheet | 702-rbcph.pdf |
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