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Home > Products > 288 003

EPS8 antibody - 288 003 K.O.

EPS8 is an actin binding regulatory protein
Rabbit polyclonal purified antibody
Cat. No.: 288 003
Amount: 50 µg
Price: $375.00
Cat. No. 288 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 648 to 821 from mouse EPS8 (UniProt Id: Q08509)
Reactivity Reacts with: rat (F1M3L7), mouse (Q08509).
Other species not tested yet.
Specificity K.O. validated
Data sheet 288_003.pdf
Cat. No.: 288 003
Amount: 50 µg
Price: $375.00
WB
ICC
IHC
Background
Actin dynamics are controlled by ensembles of actin-binding proteins. These processes include binding and/or sequestering of actin monomers, nucleation of actin filaments, capping or anticapping of barbed ends, and severing, bundling, and anchoring of F-actin (filamentous actin).
The epidermal growth factor receptor pathway substrate 8 (EPS 8) belongs to a family of proteins that is involved in the signal-transduction from Ras to Rac mediating actin remodeling. It contains a proline-rich domain, a phosphotyrosine binding (PTB) domain, an SH3-binding domain and directly caps barbed actin ends and cross-links actin filaments.