Synaptic Systems

Cat. No.: 358 103

Amount: 50 µg

Price: $375.00

Cat. No. 358 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 gallery IP: yes ICC: 1 : 1000 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 273 to 508 from human EPOR (UniProt Id: P19235)
Reactivity	Reacts with: human (P19235), mouse (P14753). Other species not tested yet.
Data sheet	358_103.pdf

References for EPOR - 358 103

Widespread expression of erythropoietin receptor in brain and its induction by injury.
Ott C, Martens H, Hassouna I, Oliveira B, Erck C, Zafeiriou MP, Peteri UK, Hesse D, Gerhart S, Altas B, Kolbow T, et al.
Molecular medicine (Cambridge, Mass.) (2015) : . 358 103 WB, IHC, IHC-P

Revisiting adult neurogenesis and the role of erythropoietin for neuronal and oligodendroglial differentiation in the hippocampus.
Hassouna I, Ott C, Wüstefeld L, Offen N, Neher RA, Mitkovski M, Winkler D, Sperling S, Fries L, Goebbels S, Vreja IC, et al.
Molecular psychiatry (2016) 2112: 1752-1767. 358 103 IHC

Cat. No.: 358 103

Amount: 50 µg

Price: $375.00

ICC

Background

The erythropoietin receptor EPOR mediates EPO-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes and triggers the JAK2/STAT5 signaling cascade.
EPO has also been shown to exert potent neuroprotective, neuroregenerative and procognitive functions suggesting EPOR expression in the brain.

1 ) Cervellini I et al., Mol. Med. (2013)
2 ) Chin H et al., Blood (1996)
3 ) Barber DL et al., Mol. Cell. Biol. (1994)

Protocols

Standard protocol

ICC

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EPOR antibody - 358 103

References for EPOR - 358 103