EPOR antibody - 358 011

EPOR is the receptor for erythropoietin

Mouse monoclonal purified IgG

Cat. No.: 358 011

Amount: 100 µg

Price: $415.00

Cat. No. 358 011	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: yes (see remarks) IP: yes ICC: 1 : 1000 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	45A3
Subtype	IgG2b (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 25 to 39 from human EPOR (UniProt Id: P19235)
Reactivity	Reacts with: human (P19235). Other species not tested yet.
Remarks	WB: Less sensitive compared to the rabbit polyclonal antibody (cat. no. 358 003). Only detects EPOR in cells with high expression levels.
Data sheet	358_011.pdf

References for EPOR - 358 011

Widespread expression of erythropoietin receptor in brain and its induction by injury.
Ott C, Martens H, Hassouna I, Oliveira B, Erck C, Zafeiriou MP, Peteri UK, Hesse D, Gerhart S, Altas B, Kolbow T, et al.
Molecular medicine (Cambridge, Mass.) (2015) : . 358 011 ICC

Cat. No.: 358 011

Amount: 100 µg

Price: $415.00

ICC

Background

The erythropoietin receptor EPOR mediates EPO-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes and triggers the JAK2/STAT5 signaling cascade.
EPO has also been shown to exert potent neuroprotective, neuroregenerative and procognitive functions suggesting EPOR expression in the brain.

1 ) Cervellini I et al., Mol. Med. (2013)
2 ) Chin H et al., Blood (1996)
3 ) Barber DL et al., Mol. Cell. Biol. (1994)

Protocols

Standard protocol

ICC

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