Synaptic Systems

Cat. No.: 276 103

Amount: 50 µg

Price: $375.00

Cat. No. 276 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 5 to 100 from mouse 4.1N (UniProt Id: Q9Z2H5)
Reactivity	Reacts with: rat (Q9WTP0), mouse (Q9Z2H5). Other species not tested yet.
Data sheet	276_103.pdf

References for EPB41L1 - 276 103

Subunit-dependent and subunit-independent rules of AMPA receptor trafficking during chemical long-term depression in hippocampal neurons.
Matsuda S, Yuzaki M
The Journal of biological chemistry (2021) 2972: 100949. 276 103 WB, IP; tested species: mouse

Cat. No.: 276 103

Amount: 50 µg

Price: $375.00

ICC

IHC

Background

The protein 4.1 family consists of a group of skeletal proteins related to the red blood cell (RBC) protein 4.1R, also known as the erythrocyte membrane skeletal band 4.1 protein EPB 41. This protein family also includes 4.1N (EPB 4), 4.1B and 4.1G (EPB 4.1L2).
4.1N and 4.1G are expressed in neuronal and non-neuronal cells in the brain. For 4.1N several splice variants with tissue specific expression patterns have been described. The 135 kDa isoform is most prominent in brain whereas a smaller 100 kDa variant is enriched in peripheral tissues

1 ) Ji Z et al., Exp Ther Med (2012)
2 ) Lin DT et al., Nat. Neurosci. (2009)
3 ) Wozny C et al., J. Cell. Sci. (2009)
4 ) Rose M et al., J. Neurochem. (2008)
5 ) Douyard J et al., J. Comp. Neurol. (2007)
6 ) Scott C et al., Eur. J. Biochem. (2001)
7 ) Kontrogianni-Konstantopoulos A et al., Mol. Biol. Cell (2000)
8 ) Walensky LD et al., J. Neurosci. (1999)

Protocols

Standard protocol

ICC

IHC

EPB41L1 (4.1N) antibody - 276 103

References for EPB41L1 - 276 103