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Home > Products > 159 004

Endophilin1 antibody - 159 004

Endophilin1 is an SH3 domain protein involved in clathrin mediated endocytosis
Guinea pig polyclonal antiserum
Cat. No.: 159 004
Amount: 100 µl
Price: $365.00
Cat. No. 159 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 256 to 276 from mouse Endophilin1 (UniProt Id: Q62420)
Reactivity Reacts with: rat, mouse.
Other species not tested yet.
Matching control protein/peptide 159-0P
Data sheet 159_004.pdf

References for Endophilin1 - 159 004

Compartmentalization of soluble endocytic proteins in synaptic vesicle clusters by phase separation.
Yoshida T, Takenaka KI, Sakamoto H, Kojima Y, Sakano T, Shibayama K, Nakamura K, Hanawa-Suetsugu K, Mori Y, Hirabayashi Y, Hirose K, et al.
iScience (2023) 266: 106826. 159 004 WB, ICC; tested species: mouse
Compartmentalization of soluble endocytic proteins in synaptic vesicle clusters by phase separation.
Yoshida T, Takenaka KI, Sakamoto H, Kojima Y, Sakano T, Shibayama K, Nakamura K, Hanawa-Suetsugu K, Mori Y, Hirabayashi Y, Hirose K, et al.
iScience (2023) 266: 106826. 159 004 WB, ICC; tested species: mouse
Cat. No.: 159 004
Amount: 100 µl
Price: $365.00
Compartmentalization of soluble endocytic proteins in synaptic vesicle clusters by phase separation.
Yoshida T, Takenaka KI, Sakamoto H, Kojima Y, Sakano T, Shibayama K, Nakamura K, Hanawa-Suetsugu K, Mori Y, Hirabayashi Y, Hirose K, et al.
iScience (2023) 266: 106826. 159 004 WB, ICC; tested species: mouse
Compartmentalization of soluble endocytic proteins in synaptic vesicle clusters by phase separation.
Yoshida T, Takenaka KI, Sakamoto H, Kojima Y, Sakano T, Shibayama K, Nakamura K, Hanawa-Suetsugu K, Mori Y, Hirabayashi Y, Hirose K, et al.
iScience (2023) 266: 106826. 159 004 WB, ICC; tested species: mouse
WB
ICC
IHC
IHC-P
Background

Endophilins are SH3 domain proteins involved in endocytosis. Both, Endophilin 1 and 2 have been shown to play important roles in clathrin mediated synaptic vesicle recycling. They recruite and stabilize the polyphosphoinositide phosphatase synaptojanin at nerve terminals.

The divergent C-terminal tail of VgluT1 has been reported to be a binding partner of Endophilin A1.

In contrast to Endophilin 1 that shows a brain specific expression, Endophilin 2 is abundantly expressed in different tissues.
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