Synaptic Systems

Cat. No.: 143 004

Amount: 100 µl

Price: $365.00

WB

ICC

Background

ELKS, also referred to as ERCs (ERC 1 and ERC 2) and CAST, are related proteins which share an identical C-terminal sequence. They interact with the conserved RIM PDZ domain via an unusual PDZ binding motif. Two splice variants of ERC 1 (1a and 1b) have been described. ERC 1b (CAST 2a) binds to RIM and is expressed exclusively in the brain. ERC 1a is a ubiquitously expressed cytosolic protein. ERC 2 (CAST 1) is only expressed as a single RIM binding variant.
All ERCs have been shown to interact with Rab 6, a protein involved in membrane trafficking at the Golgi complex. The function of these proteins has not been determined yet. They may link Rab 6 mediated non-neuronal membrane traffic at the Golgi complex to neuronal membrane traffic at the active zone executed via RIMs.

1 ) Takao-Rikitsu E et al., J. Cell Biol. (2004)
2 ) Deguchi-Tawarada M et al., Genes Cells (2004)
3 ) Ko J et al., J. Biol. Chem. (2003)
4 ) Ohtsuka T et al., J. Cell Biol. (2002)
5 ) Wang Y et al., Proc. Natl. Acad. Sci. U.S.A. (2002)

Protocols

WB

ICC

OTHER

Preabsorbtion

Other products for this target

Cat. No. 143 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 939 to 948 from rat Erc1b (UniProt Id: Q811U3-1)
Reactivity	Reacts with: human (Q8IUD2, O15083), rat (Q811U3, Q8K3M6), mouse (Q99MI1, Q6PH08). Other species not tested yet.
Specificity	Specific for the brain specific isoforms ERC 1b and ERC 2.
Matching control protein/peptide	143-0P
Data sheet	143_004.pdf

ERC1b/2 antibody - 143 004