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Home > Products > 143 003

ERC1b/2 antibody - 143 003

ERCs are RIM binding proteins
Rabbit polyclonal purified antibody
Cat. No.: 143 003
Amount: 50 µg
Price: $375.00
Cat. No. 143 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 1000 gallery  
IHC: 1 : 100 gallery  
IHC-P: not tested yet
Immunogen Synthetic peptide corresponding to AA 939 to 948 from rat Erc1b (UniProt Id: Q811U3-1)
Reactivity Reacts with: human (Q8IUD2, O15083), rat (Q811U3, Q8K3M6), mouse (Q99MI1, Q6PH08), hamster.
No signal: zebrafish.
Other species not tested yet.
Specificity Specific for the brain specific isoforms ERC 1b and ERC 2.
Matching control protein/peptide 143-0P
Data sheet 143_003.pdf

References for ERC1b/2 - 143 003

Liprin-α proteins are master regulators of human presynapse assembly.
Marcó de la Cruz B, Campos J, Molinaro A, Xie X, Jin G, Wei Z, Acuna C, Sterky FH
Nature neuroscience (2024) : . 143 003 WB, ICC; tested species: human
A presynaptic phosphosignaling hub for lasting homeostatic plasticity.
Müller JA, Betzin J, Santos-Tejedor J, Mayer A, Oprişoreanu AM, Engholm-Keller K, Paulußen I, Gulakova P, McGovern TD, Gschossman LJ, Schönhense E, et al.
Cell reports (2022) 393: 110696. 143 003 WB, ICC; tested species: mouse
Syndapin I Loss-of-Function in Mice Leads to Schizophrenia-Like Symptoms.
Koch N, Koch D, Krueger S, Tröger J, Sabanov V, Ahmed T, McMillan LE, Wolf D, Montag D, Kessels MM, Balschun D, et al.
Cerebral cortex (New York, N.Y. : 1991) (2020) : . 143 003 WB; tested species: mouse
α2δ-4 is required for the molecular and structural organization of rod and cone photoreceptor synapses.
Kerov V, Laird JG, Joiner ML, Knecht S, Soh D, Hagen J, Gardner SH, Gutierrez W, Yoshimatsu T, Bhattarai S, Puthussery T, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 143 003 WB; tested species: mouse
The active zone protein CAST regulates synaptic vesicle recycling and quantal size in the mouse hippocampus.
Kobayashi S, Hida Y, Ishizaki H, Inoue E, Tanaka-Okamoto M, Yamasaki M, Miyazaki T, Fukaya M, Kitajima I, Takai Y, Watanabe M, et al.
The European journal of neuroscience (2016) 445: 2272-84. 143 003 WB
RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses.
Grauel MK, Maglione M, Reddy-Alla S, Willmes CG, Brockmann MM, Trimbuch T, Rosenmund T, Pangalos M, Vardar G, Stumpf A, Walter AM, et al.
Proceedings of the National Academy of Sciences of the United States of America (2016) 11341: 11615-11620. 143 003 WB; tested species: mouse
Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 143 003 WB, ICC
RIM1α SUMOylation is required for fast synaptic vesicle exocytosis.
Girach F, Craig TJ, Rocca DL, Henley JM
Cell reports (2013) 55: 1294-301. 143 003 WB; tested species: rat
Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.
Spangler SA, Schmitz SK, Kevenaar JT, de Graaff E, de Wit H, Demmers J, Toonen RF, Hoogenraad CC
The Journal of cell biology (2013) 2016: 915-28. 143 003 ICC, WB; tested species: rat
Mover is a homomeric phospho-protein present on synaptic vesicles.
Ahmed S, Wittenmayer N, Kremer T, Hoeber J, Kiran Akula A, Urlaub H, Islinger M, Kirsch J, Dean C, Dresbach T
PloS one (2013) 85: e63474. 143 003 WB
Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 143 003 WB; tested species: mouse
mSYD1A, a mammalian synapse-defective-1 protein, regulates synaptogenic signaling and vesicle docking.
Wentzel C, Sommer JE, Nair R, Stiefvater A, Sibarita JB, Scheiffele P
Neuron (2013) 786: 1012-23. 143 003 WB; tested species: mouse
Formation of Golgi-derived active zone precursor vesicles.
Maas C, Torres VI, Altrock WD, Leal-Ortiz S, Wagh D, Terry-Lorenzo RT, Fejtova A, Gundelfinger ED, Ziv NE, Garner CC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3232: 11095-108. 143 003 WB
Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing.
Lazarevic V, Schöne C, Heine M, Gundelfinger ED, Fejtova A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10189-200. 143 003 WB, ICC
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 143 003 WB
Molecular dissection of the photoreceptor ribbon synapse: physical interaction of Bassoon and RIBEYE is essential for the assembly of the ribbon complex.
tom Dieck S, Altrock WD, Kessels MM, Qualmann B, Regus H, Brauner D, Fejtová A, Bracko O, Gundelfinger ED, Brandstätter JH
The Journal of cell biology (2005) 1685: 825-36. 143 003 WB, IHC
Liprin-α proteins are master regulators of human presynapse assembly.
Marcó de la Cruz B, Campos J, Molinaro A, Xie X, Jin G, Wei Z, Acuna C, Sterky FH
Nature neuroscience (2024) : . 143 003 WB, ICC; tested species: human
A presynaptic phosphosignaling hub for lasting homeostatic plasticity.
Müller JA, Betzin J, Santos-Tejedor J, Mayer A, Oprişoreanu AM, Engholm-Keller K, Paulußen I, Gulakova P, McGovern TD, Gschossman LJ, Schönhense E, et al.
Cell reports (2022) 393: 110696. 143 003 WB, ICC; tested species: mouse
Synaptic weight set by Munc13-1 supramolecular assemblies.
Sakamoto H, Ariyoshi T, Kimpara N, Sugao K, Taiko I, Takikawa K, Asanuma D, Namiki S, Hirose K
Nature neuroscience (2018) 211: 41-49. 143 003 ICC; tested species: rat
Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 143 003 WB, ICC
Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.
Spangler SA, Schmitz SK, Kevenaar JT, de Graaff E, de Wit H, Demmers J, Toonen RF, Hoogenraad CC
The Journal of cell biology (2013) 2016: 915-28. 143 003 ICC, WB; tested species: rat
Lateral mobility of presynaptic α7-containing nicotinic receptors and its relevance for glutamate release.
Gomez-Varela D, Berg DK
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3343: 17062-71. 143 003 ICC; tested species: rat
Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing.
Lazarevic V, Schöne C, Heine M, Gundelfinger ED, Fejtova A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10189-200. 143 003 WB, ICC
Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue.
Vatan T, Minehart JA, Zhang C, Agarwal V, Yang J, Speer CM
STAR protocols (2021) 24: 100971. 143 003 IHC; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 143 003 IHC; tested species: rat
ELKS1 and Ca(2+) channel subunit β4 interact and colocalize at cerebellar synapses.
Billings SE, Clarke GL, Nishimune H
Neuroreport (2012) 231: 49-54. 143 003 IHC
Active zone proteins are dynamically associated with synaptic ribbons in rat pinealocytes.
Spiwoks-Becker I, Maus C, tom Dieck S, Fejtová A, Engel L, Wolloscheck T, Wolfrum U, Vollrath L, Spessert R
Cell and tissue research (2008) 3332: 185-95. 143 003 IHC; tested species: rat
Molecular dissection of the photoreceptor ribbon synapse: physical interaction of Bassoon and RIBEYE is essential for the assembly of the ribbon complex.
tom Dieck S, Altrock WD, Kessels MM, Qualmann B, Regus H, Brauner D, Fejtová A, Bracko O, Gundelfinger ED, Brandstätter JH
The Journal of cell biology (2005) 1685: 825-36. 143 003 WB, IHC
Cat. No.: 143 003
Amount: 50 µg
Price: $375.00
Liprin-α proteins are master regulators of human presynapse assembly.
Marcó de la Cruz B, Campos J, Molinaro A, Xie X, Jin G, Wei Z, Acuna C, Sterky FH
Nature neuroscience (2024) : . 143 003 WB, ICC; tested species: human
A presynaptic phosphosignaling hub for lasting homeostatic plasticity.
Müller JA, Betzin J, Santos-Tejedor J, Mayer A, Oprişoreanu AM, Engholm-Keller K, Paulußen I, Gulakova P, McGovern TD, Gschossman LJ, Schönhense E, et al.
Cell reports (2022) 393: 110696. 143 003 WB, ICC; tested species: mouse
Syndapin I Loss-of-Function in Mice Leads to Schizophrenia-Like Symptoms.
Koch N, Koch D, Krueger S, Tröger J, Sabanov V, Ahmed T, McMillan LE, Wolf D, Montag D, Kessels MM, Balschun D, et al.
Cerebral cortex (New York, N.Y. : 1991) (2020) : . 143 003 WB; tested species: mouse
α2δ-4 is required for the molecular and structural organization of rod and cone photoreceptor synapses.
Kerov V, Laird JG, Joiner ML, Knecht S, Soh D, Hagen J, Gardner SH, Gutierrez W, Yoshimatsu T, Bhattarai S, Puthussery T, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 143 003 WB; tested species: mouse
The active zone protein CAST regulates synaptic vesicle recycling and quantal size in the mouse hippocampus.
Kobayashi S, Hida Y, Ishizaki H, Inoue E, Tanaka-Okamoto M, Yamasaki M, Miyazaki T, Fukaya M, Kitajima I, Takai Y, Watanabe M, et al.
The European journal of neuroscience (2016) 445: 2272-84. 143 003 WB
RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses.
Grauel MK, Maglione M, Reddy-Alla S, Willmes CG, Brockmann MM, Trimbuch T, Rosenmund T, Pangalos M, Vardar G, Stumpf A, Walter AM, et al.
Proceedings of the National Academy of Sciences of the United States of America (2016) 11341: 11615-11620. 143 003 WB; tested species: mouse
Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 143 003 WB, ICC
RIM1α SUMOylation is required for fast synaptic vesicle exocytosis.
Girach F, Craig TJ, Rocca DL, Henley JM
Cell reports (2013) 55: 1294-301. 143 003 WB; tested species: rat
Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.
Spangler SA, Schmitz SK, Kevenaar JT, de Graaff E, de Wit H, Demmers J, Toonen RF, Hoogenraad CC
The Journal of cell biology (2013) 2016: 915-28. 143 003 ICC, WB; tested species: rat
Mover is a homomeric phospho-protein present on synaptic vesicles.
Ahmed S, Wittenmayer N, Kremer T, Hoeber J, Kiran Akula A, Urlaub H, Islinger M, Kirsch J, Dean C, Dresbach T
PloS one (2013) 85: e63474. 143 003 WB
Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 143 003 WB; tested species: mouse
mSYD1A, a mammalian synapse-defective-1 protein, regulates synaptogenic signaling and vesicle docking.
Wentzel C, Sommer JE, Nair R, Stiefvater A, Sibarita JB, Scheiffele P
Neuron (2013) 786: 1012-23. 143 003 WB; tested species: mouse
Formation of Golgi-derived active zone precursor vesicles.
Maas C, Torres VI, Altrock WD, Leal-Ortiz S, Wagh D, Terry-Lorenzo RT, Fejtova A, Gundelfinger ED, Ziv NE, Garner CC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3232: 11095-108. 143 003 WB
Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing.
Lazarevic V, Schöne C, Heine M, Gundelfinger ED, Fejtova A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10189-200. 143 003 WB, ICC
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 143 003 WB
Molecular dissection of the photoreceptor ribbon synapse: physical interaction of Bassoon and RIBEYE is essential for the assembly of the ribbon complex.
tom Dieck S, Altrock WD, Kessels MM, Qualmann B, Regus H, Brauner D, Fejtová A, Bracko O, Gundelfinger ED, Brandstätter JH
The Journal of cell biology (2005) 1685: 825-36. 143 003 WB, IHC
Liprin-α proteins are master regulators of human presynapse assembly.
Marcó de la Cruz B, Campos J, Molinaro A, Xie X, Jin G, Wei Z, Acuna C, Sterky FH
Nature neuroscience (2024) : . 143 003 WB, ICC; tested species: human
A presynaptic phosphosignaling hub for lasting homeostatic plasticity.
Müller JA, Betzin J, Santos-Tejedor J, Mayer A, Oprişoreanu AM, Engholm-Keller K, Paulußen I, Gulakova P, McGovern TD, Gschossman LJ, Schönhense E, et al.
Cell reports (2022) 393: 110696. 143 003 WB, ICC; tested species: mouse
Synaptic weight set by Munc13-1 supramolecular assemblies.
Sakamoto H, Ariyoshi T, Kimpara N, Sugao K, Taiko I, Takikawa K, Asanuma D, Namiki S, Hirose K
Nature neuroscience (2018) 211: 41-49. 143 003 ICC; tested species: rat
Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 143 003 WB, ICC
Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.
Spangler SA, Schmitz SK, Kevenaar JT, de Graaff E, de Wit H, Demmers J, Toonen RF, Hoogenraad CC
The Journal of cell biology (2013) 2016: 915-28. 143 003 ICC, WB; tested species: rat
Lateral mobility of presynaptic α7-containing nicotinic receptors and its relevance for glutamate release.
Gomez-Varela D, Berg DK
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3343: 17062-71. 143 003 ICC; tested species: rat
Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing.
Lazarevic V, Schöne C, Heine M, Gundelfinger ED, Fejtova A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10189-200. 143 003 WB, ICC
Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue.
Vatan T, Minehart JA, Zhang C, Agarwal V, Yang J, Speer CM
STAR protocols (2021) 24: 100971. 143 003 IHC; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 143 003 IHC; tested species: rat
ELKS1 and Ca(2+) channel subunit β4 interact and colocalize at cerebellar synapses.
Billings SE, Clarke GL, Nishimune H
Neuroreport (2012) 231: 49-54. 143 003 IHC
Active zone proteins are dynamically associated with synaptic ribbons in rat pinealocytes.
Spiwoks-Becker I, Maus C, tom Dieck S, Fejtová A, Engel L, Wolloscheck T, Wolfrum U, Vollrath L, Spessert R
Cell and tissue research (2008) 3332: 185-95. 143 003 IHC; tested species: rat
Molecular dissection of the photoreceptor ribbon synapse: physical interaction of Bassoon and RIBEYE is essential for the assembly of the ribbon complex.
tom Dieck S, Altrock WD, Kessels MM, Qualmann B, Regus H, Brauner D, Fejtová A, Bracko O, Gundelfinger ED, Brandstätter JH
The Journal of cell biology (2005) 1685: 825-36. 143 003 WB, IHC
WB
ICC
IHC
Background

ELKS, also referred to as ERCs (ERC 1 and ERC 2) and CAST, are related proteins which share an identical C-terminal sequence. They interact with the conserved RIM PDZ domain via an unusual PDZ binding motif. Two splice variants of ERC 1 (1a and 1b) have been described. ERC 1b (CAST 2a) binds to RIM and is expressed exclusively in the brain. ERC 1a is a ubiquitously expressed cytosolic protein. ERC 2 (CAST 1) is only expressed as a single RIM binding variant.
All ERCs have been shown to interact with Rab 6, a protein involved in membrane trafficking at the Golgi complex. The function of these proteins has not been determined yet. They may link Rab 6 mediated non-neuronal membrane traffic at the Golgi complex to neuronal membrane traffic at the active zone executed via RIMs.
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