Synaptic Systems

Cat. No.: 237 105

Amount: 50 µg

Price: $465.00

Cat. No. 237 105	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 AP staining gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1 to 15 from mouse EEA1 (UniProt Id: Q8BL66)
Reactivity	Reacts with: mouse (Q8BL66), rat (A0A0G2K051). Other species not tested yet.
Data sheet	237_105.pdf

References for EEA1 - 237 105

The infantile myofibromatosis NOTCH3 L1519P mutation leads to hyperactivated ligand-independent Notch signaling and increased PDGFRB expression.
Wu D, Wang S, Oliveira DV, Del Gaudio F, Vanlandewijck M, Lebouvier T, Betsholtz C, Zhao J, Jin S, Lendahl U, Karlström H, et al.
Disease models & mechanisms (2021) : . 237 105 ICC; tested species: human

A Super-Resolved View of the Alzheimer's Disease-Related Amyloidogenic Pathway in Hippocampal Neurons.
Yu Y, Gao Y, Winblad B, Tjernberg LO, Schedin-Weiss S
Journal of Alzheimer's disease : JAD (2021) 832: 833-852. 237 105 ICC; tested species: mouse

Cat. No.: 237 105

Amount: 50 µg

Price: $465.00

ICC

IHC

Background

Extracellular compounds are internalized by endocytosis into so called endocytic vesicles. They fuse with early endosomes, from where the endocytosed material can be shuttled to a number of alternative destinations.
Early endosomal antigen 1 (EEA 1) is a peripheral membrane protein that locates to early endosomes via binding to the membrane lipid phosphatidylinositol 3-phosphate (PtdIns3P) and the active form of Rab5.
Autoantibodies against EEA 1 have been shown to be associated with subacute cutaneous systemic lupus erythematosus.

1 ) Bergeland T et al., EMBO Rep. (2008)
2 ) Wilson JM et al., Mol. Biol. Cell (2000)
3 ) Simonsen A et al., J. Biol. Chem. (1999)
4 ) Callaghan J et al., Biochem. J. (1999)
5 ) Stenmark H et al., J. Biol. Chem. (1996)
6 ) Mu FT et al., J. Biol. Chem. (1995)

Protocols

ICC

IHC

Other products for this target

EEA1 antibody - 237 105

References for EEA1 - 237 105