EEA1 peptide - 237-0P

EEA1 is an endosomal Rab 5 effector essential for endocytic membrane fusion

control peptide

Cat. No.: 237-0P

Amount: 100 µg

Price: $105.00

Cat. No. 237-0P	100 µg peptide, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized!
Applications
Immunogen	Synthetic peptide corresponding to AA 2 to 13 from rat EEA1 (UniProt Id: A0A0G2K051)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (aa 2 - 13 in rodent EEA 1) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	237-0p.pdf

Cat. No.: 237-0P

Amount: 100 µg

Price: $105.00

Background

Extracellular compounds are internalized by endocytosis into so called endocytic vesicles. They fuse with early endosomes, from where the endocytosed material can be shuttled to a number of alternative destinations.
Early endosomal antigen 1 (EEA 1) is a peripheral membrane protein that locates to early endosomes via binding to the membrane lipid phosphatidylinositol 3-phosphate (PtdIns3P) and the active form of Rab5.
Autoantibodies against EEA 1 have been shown to be associated with subacute cutaneous systemic lupus erythematosus.

1 ) Bergeland T et al., EMBO Rep. (2008)
2 ) Wilson JM et al., Mol. Biol. Cell (2000)
3 ) Simonsen A et al., J. Biol. Chem. (1999)
4 ) Callaghan J et al., Biochem. J. (1999)
5 ) Stenmark H et al., J. Biol. Chem. (1996)
6 ) Mu FT et al., J. Biol. Chem. (1995)

Protocols

OTHER

Preadsorbtion

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