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EAAT3 antibody cytoplasmic domain - currently not available - 250 303

Polyclonal antibodies are a finite resource. We spend a lot of effort to produce and validate new batches to ensure consistent performance. Sometimes this is not immediately successful, so that temporary supply problems can occur.
EAATs are transmembrane proteins involved in the removal of extracellular glutamate
Rabbit polyclonal purified antibody
Cat. No.: 250 303
Amount: 50 µg
Price: $375.00
Cat. No. 250 303 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: 1 : 100 up to 1 : 500 gallery  
IHC-P: not tested yet
Immunogen Synthetic peptide corresponding to AA 487 to 500 from rat EAAT3 (UniProt Id: P51907)
Reactivity Reacts with: rat (P51907), mouse (P51906).
Other species not tested yet.
Data sheet 250_303.pdf
Cat. No.: 250 303
Amount: 50 µg
Price: $375.00
Background

Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. After the release of glutamate from synaptic vesicles into the synaptic cleft during neurotransmission, excitatory amino acid transporters (EAATs) remove extracellular glutamate to avoid excitotoxic levels (1).
Five EAATs with differential expression patterns have been described so far: EAAT1, also referred to as GLAST and SLC1A3, has neuroprotective potential following ischemia and occurs in reactive astrocytes and activated microglia. EAAT2 (GLT-1, SLC1A2) is the most abundant isoform and is primarily expressed in astrocytes. Both variants show high levels in brain and retina. EAAT3 / SLC1A1, EAAT4 / SLC1A6 and EAAT5 / SLC1A7 are expressed in neurons (2). EAAT4 shows weak expression in the forebrain and high levels in the cerebellum (3). EAAT5 primarily occurs in the retina where it locates very close to glutamate release sites. In K.O. mice flicker resolution is considerably compromised (4). Recent findings suggest that EAAT5 is an abundant isoform, expressed also in non-neuronal peripheral tissues (5).