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EAAT1 antibody cytoplasmic domain - 250 114

EAATs are transmembrane proteins involved in the removal of extracellular glutamate
Guinea pig polyclonal antiserum
Cat. No.: 250 114
Amount: 100 µl
Price: $365.00
Cat. No. 250 114 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 500 gallery  
Immunogen Synthetic peptide corresponding to AA 522 to 541 from rat EAAT1 (UniProt Id: P24942)
Reactivity Reacts with: rat (P24942), mouse.
Other species not tested yet.
Matching control protein/peptide 250-11P
Data sheet 250_114.pdf

References for EAAT1 - 250 114

The expression and function of glutamate aspartate transporters in Bergmann glia are decreased in neuronal nitric oxide synthase-knockout mice during postnatal development.
Tellios V, Maksoud MJE, Lu WY
Glia (2022) : . 250 114 WB, IHC; tested species: mouse
Loss of excitatory amino acid transporter restraint following chronic intermittent hypoxia contribute to synaptic alterations in nucleus tractus solitarii.
Martinez D, Rogers RC, Hasser EM, Hermann GE, Kline DD
Journal of neurophysiology (2020) : . 250 114 WB; tested species: rat
The expression and function of glutamate aspartate transporters in Bergmann glia are decreased in neuronal nitric oxide synthase-knockout mice during postnatal development.
Tellios V, Maksoud MJE, Lu WY
Glia (2022) : . 250 114 WB, IHC; tested species: mouse
Neuroepithelial progenitors generate and propagate non-neuronal action potentials across the spinal cord.
Arulkandarajah KH, Osterstock G, Lafont A, Le Corronc H, Escalas N, Corsini S, Le Bras B, Chenane L, Boeri J, Czarnecki A, Mouffle C, et al.
Current biology : CB (2021) 3120: 4584-4595.e4. 250 114 IHC; tested species: mouse
Cannabinoid Type 1 Receptor is Undetectable in Rodent and Primate Cerebral Neural Stem Cells but Participates in Radial Neuronal Migration.
Morozov YM, Mackie K, Rakic P
International journal of molecular sciences (2020) 2122: . 250 114 IHC; tested species: mouse
Cat. No.: 250 114
Amount: 100 µl
Price: $365.00
The expression and function of glutamate aspartate transporters in Bergmann glia are decreased in neuronal nitric oxide synthase-knockout mice during postnatal development.
Tellios V, Maksoud MJE, Lu WY
Glia (2022) : . 250 114 WB, IHC; tested species: mouse
Loss of excitatory amino acid transporter restraint following chronic intermittent hypoxia contribute to synaptic alterations in nucleus tractus solitarii.
Martinez D, Rogers RC, Hasser EM, Hermann GE, Kline DD
Journal of neurophysiology (2020) : . 250 114 WB; tested species: rat
The expression and function of glutamate aspartate transporters in Bergmann glia are decreased in neuronal nitric oxide synthase-knockout mice during postnatal development.
Tellios V, Maksoud MJE, Lu WY
Glia (2022) : . 250 114 WB, IHC; tested species: mouse
Neuroepithelial progenitors generate and propagate non-neuronal action potentials across the spinal cord.
Arulkandarajah KH, Osterstock G, Lafont A, Le Corronc H, Escalas N, Corsini S, Le Bras B, Chenane L, Boeri J, Czarnecki A, Mouffle C, et al.
Current biology : CB (2021) 3120: 4584-4595.e4. 250 114 IHC; tested species: mouse
Cannabinoid Type 1 Receptor is Undetectable in Rodent and Primate Cerebral Neural Stem Cells but Participates in Radial Neuronal Migration.
Morozov YM, Mackie K, Rakic P
International journal of molecular sciences (2020) 2122: . 250 114 IHC; tested species: mouse
Background

Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. After the release of glutamate from synaptic vesicles into the synaptic cleft during neurotransmission, excitatory amino acid transporters (EAATs) remove extracellular glutamate to avoid excitotoxic levels (1).
Five EAATs with differential expression patterns have been described so far: EAAT1, also referred to as GLAST and SLC1A3, has neuroprotective potential following ischemia and occurs in reactive astrocytes and activated microglia. EAAT2 (GLT-1, SLC1A2) is the most abundant isoform and is primarily expressed in astrocytes. Both variants show high levels in brain and retina. EAAT3 / SLC1A1, EAAT4 / SLC1A6 and EAAT5 / SLC1A7 are expressed in neurons (2). EAAT4 shows weak expression in the forebrain and high levels in the cerebellum (3). EAAT5 primarily occurs in the retina where it locates very close to glutamate release sites. In K.O. mice flicker resolution is considerably compromised (4). Recent findings suggest that EAAT5 is an abundant isoform, expressed also in non-neuronal peripheral tissues (5).