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Home > Products > 115 303

Dynamin3 antibody - 115 303

Dynamins are GTPases involved in endocytosis
Rabbit polyclonal purified antibody
Cat. No.: 115 303
Amount: 50 µg
Price: $455.00
Cat. No. 115 303 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
Immunogen Synthetic peptide corresponding to AA 773 to 794 from mouse Dynamin3 (UniProt Id: Q8BZ98)
Reactivity Reacts with: rat (Q08877), mouse (Q8BZ98).
Other species not tested yet.
Specificity Detects dynamin 3 with weak cross-reactivity for Dynamin1
Matching control protein/peptide 115-3P
Data sheet 115_303.pdf
Cat. No.: 115 303
Amount: 50 µg
Price: $455.00
WB
ICC
IHC
IHC-P
Background
Dynamin was discovered because of its binding to microtubules. It was later shown not to function in the cytoskeleton but in endocytosis. Dynamin is required for clathrin - mediated endocytosis. It contains a NH2 - terminal GTPase domain, a middle pleckstrin - homology domain, and a COOH- terminal proline - rich sequence. The COOH - terminal sequence binds to amphiphilin which contains a SH3 domain that recognizes the proline - rich sequence of dynamin.
There are at least three isoforms of dynamin: Dynamin 1 is enriched in synapses whereas dynamin 2 is ubiquitous and dynamin 3 is expressed in brain and testis. Neuronal dynamin 1 is phosphorylated by protein kinase C and dephosphorylated by calcineurin during an action potential in the nerve terminal. It is possible that the dephosphorylation provides a trigger for endocytosis.
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