Cat. No.: 115 002
Amount: 200 µl
Price:
$355.00
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| Cat. No. 115 002 |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Immunogen | Synthetic peptide corresponding to AA 2 to 17 from rat Dynamin1 (UniProt Id: P21575) |
| Reactivity |
Reacts with: human (Q05193, P50570, Q9UQ16), rat (P21575, P39052, Q08877), mouse (P39053, P39054, Q8BZ98), cow. Other species not tested yet. |
| Specificity | Recognizes dynamin 1, 2 and 3 with a preference for dynamin 1. K.D. validated PubMed: 32989096 |
| Matching control protein/peptide | 115-0P |
| Data sheet | 115_002.pdf |
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Dynamin was discovered because of its binding to microtubules. It was later shown not to function in the cytoskeleton but in endocytosis. Dynamin is required for clathrin - mediated endocytosis. It contains a NH2 - terminal GTPase domain, a middle pleckstrin - homology domain, and a COOH- terminal proline - rich sequence. The COOH - terminal sequence binds to amphiphilin which contains a SH3 domain that recognizes the proline - rich sequence of dynamin.
There are at least three isoforms of dynamin: Dynamin 1 is enriched in synapses whereas dynamin 2 is ubiquitous and dynamin 3 is expressed in brain and testis. Neuronal dynamin 1 is phosphorylated by protein kinase C and dephosphorylated by calcineurin during an action potential in the nerve terminal. It is possible that the dephosphorylation provides a trigger for endocytosis.