Synaptic Systems - Dynamin1/2/3

Cat. No.: 115 002

Amount: 200 µl

Price: $360.00

Cat. No. 115 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 200 up to 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 100 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 2 to 17 from rat Dynamin1 (UniProt Id: P21575)
Reactivity	Reacts with: human (Q05193, P50570, Q9UQ16), rat (P21575, P39052, Q08877), mouse (P39053, P39054, Q8BZ98), cow. Other species not tested yet.
Specificity	Recognizes dynamin 1, 2 and 3 with a preference for dynamin 1. K.D. validated PubMed: 32989096
Matching control protein/peptide	115-0P
Data sheet	115_002.pdf

References for Dynamin1/2/3 - 115 002

Endosomal phosphatidylinositol 3-phosphate controls synaptic vesicle cycling and neurotransmission.
Liu GT, Kochlamazashvili G, Puchkov D, Müller R, Schultz C, Mackintosh AI, Vollweiter D, Haucke V, Soykan T
The EMBO journal (2022) : e109352. 115 002 WB; tested species: rat

Release mode dynamically regulates the RRP refilling mechanism at individual hippocampal synapses.
Kim Y, Lee U, Choi C, Chang S
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 115 002 WB, ICC; KD verified; tested species: rat

VGLUT1 Binding to Endophilin or Intersectin1 and Dynamin Phosphorylation in a Diurnal Context.
Richter K, Schmutz I, Darna M, Zander JF, Chavan R, Albrecht U, Ahnert-Hilger G
Neuroscience (2018) 371: 29-37. 115 002 WB; tested species: mouse

Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Grønborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, et al.
The EMBO journal (2014) 332: 157-70. 115 002 WB; tested species: mouse

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 115 002 WB

Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 115 002 WB; tested species: mouse

Efficient synaptic vesicle recycling after intense exocytosis concomitant with the accumulation of non-releasable endosomes at early developmental stages.
Bartolomé-Martín D, Ramírez-Franco J, Castro E, Sánchez-Prieto J, Torres M
Journal of cell science (2012) 125Pt 2: 422-34. 115 002 WB, ICC; tested species: rat

Increased neurotransmitter release at the neuromuscular junction in a mouse model of polyglutamine disease.
Rozas JL, Gómez-Sánchez L, Tomás-Zapico C, Lucas JJ, Fernández-Chacón R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 313: 1106-13. 115 002 WB

Dynamic interaction of amphiphysin with N-WASP regulates actin assembly.
Yamada H, Padilla-Parra S, Park SJ, Itoh T, Chaineau M, Monaldi I, Cremona O, Benfenati F, De Camilli P, Coppey-Moisan M, Tramier M, et al.
The Journal of biological chemistry (2009) 28449: 34244-56. 115 002 WB

Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 115 002 WB

Distinct synaptic vesicle recycling in inhibitory nerve terminals is coordinated by SV2A.
Bae JR, Lee W, Jo YO, Han S, Koh S, Song WK, Kim SH
Progress in neurobiology (2020) : 101879. 115 002 ICC; tested species: rat

Dynamin regulates the fusion pore of endo- and exocytotic vesicles as revealed by membrane capacitance measurements.
Lasič E, Stenovec M, Kreft M, Robinson PJ, Zorec R
Biochimica et biophysica acta (2017) 18619: 2293-2303. 115 002 ICC; tested species: rat

A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis.
Ferguson SM, Brasnjo G, Hayashi M, Wölfel M, Collesi C, Giovedi S, Raimondi A, Gong LW, Ariel P, Paradise S, O'toole E, et al.
Science (New York, N.Y.) (2007) 3165824: 570-4. 115 002 ICC

Imaging direct, dynamin-dependent recapture of fusing secretory granules on plasma membrane lawns from PC12 cells.
Holroyd P, Lang T, Wenzel D, De Camilli P, Jahn R
Proceedings of the National Academy of Sciences of the United States of America (2002) 9926: 16806-11. 115 002 ICC; tested species: rat

Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 115 002

Cat. No.: 115 002

Amount: 200 µl

Price: $360.00

Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 115 002 WB; tested species: mouse

ICC

IHC

IHC-P

Background

Dynamin was discovered because of its binding to microtubules. It was later shown not to function in the cytoskeleton but in endocytosis. Dynamin is required for clathrin - mediated endocytosis. It contains a NH2 - terminal GTPase domain, a middle pleckstrin - homology domain, and a COOH- terminal proline - rich sequence. The COOH - terminal sequence binds to amphiphilin which contains a SH3 domain that recognizes the proline - rich sequence of dynamin.
There are at least three isoforms of dynamin: Dynamin 1 is enriched in synapses whereas dynamin 2 is ubiquitous and dynamin 3 is expressed in brain and testis. Neuronal dynamin 1 is phosphorylated by protein kinase C and dephosphorylated by calcineurin during an action potential in the nerve terminal. It is possible that the dephosphorylation provides a trigger for endocytosis.

1 ) Hinshaw JE et al., Annu. Rev. Cell Dev. Biol. (2000)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Slepnev VI et al., Nat. Rev. Neurosci. (2000)
4 ) Brodin L et al., Curr. Opin. Neurobiol. (2000)
5 ) Hannah MJ et al., Annu. Rev. Cell Dev. Biol. (1999)
6 ) Südhof TC et al., Nature (1995)
7 ) McMahon HT et al., Cell (1995)
8 ) Sontag JM et al., J. Biol. Chem. (1994)
9 ) Robinson PJ et al., Nature (1993)
10 ) Obar RA et al., Nature (1990)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Dynamin1/2/3 antibody - 115 002 K.D.

References for Dynamin1/2/3 - 115 002