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Doublecortin antibody - 326 006

Doublecortin or DCX is a protein associated with neuronal differentiation
Chicken polyclonal purified antibody
Cat. No.: 326 006
Amount: 50 µg
Price: $380.00
Cat. No. 326 006 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 1000 up to 1 : 2000 gallery  
IHC-P: 1 : 2000 gallery  
Immunogen Recombinant protein corresponding to AA 271 to 366 from mouse Doublecortin (UniProt Id: Q6PGI2)
Reactivity Reacts with: mouse (Q6PGI2).
Other species not tested yet.
Data sheet 326_006.pdf

References for Doublecortin - 326 006

Prolonged fixation and post-mortem delay impede the study of adult neurogenesis in mice.
Gallardo-Caballero M, Rodríguez-Moreno CB, Álvarez-Méndez L, Terreros-Roncal J, Flor-García M, Moreno-Jiménez EP, Rábano A, Llorens-Martín M
Communications biology (2023) 61: 978. 326 006 IHC; tested species: mouse
Microglial control of neuronal development via somatic purinergic junctions.
Cserép C, Schwarcz AD, Pósfai B, László ZI, Kellermayer A, Környei Z, Kisfali M, Nyerges M, Lele Z, Katona I, Ádám Dénes , et al.
Cell reports (2022) 4012: 111369. 326 006 IHC; tested species: mouse
Oxytocin signaling is necessary for synaptic maturation of adult-born neurons.
Pekarek BT, Kochukov M, Lozzi B, Wu T, Hunt PJ, Tepe B, Hanson Moss E, Tantry EK, Swanson JL, Dooling SW, Patel M, et al.
Genes & development () 3621-24: 1100-1118. 326 006 IHC; tested species: mouse
Cat. No.: 326 006
Amount: 50 µg
Price: $380.00
Prolonged fixation and post-mortem delay impede the study of adult neurogenesis in mice.
Gallardo-Caballero M, Rodríguez-Moreno CB, Álvarez-Méndez L, Terreros-Roncal J, Flor-García M, Moreno-Jiménez EP, Rábano A, Llorens-Martín M
Communications biology (2023) 61: 978. 326 006 IHC; tested species: mouse
Microglial control of neuronal development via somatic purinergic junctions.
Cserép C, Schwarcz AD, Pósfai B, László ZI, Kellermayer A, Környei Z, Kisfali M, Nyerges M, Lele Z, Katona I, Ádám Dénes , et al.
Cell reports (2022) 4012: 111369. 326 006 IHC; tested species: mouse
Oxytocin signaling is necessary for synaptic maturation of adult-born neurons.
Pekarek BT, Kochukov M, Lozzi B, Wu T, Hunt PJ, Tepe B, Hanson Moss E, Tantry EK, Swanson JL, Dooling SW, Patel M, et al.
Genes & development () 3621-24: 1100-1118. 326 006 IHC; tested species: mouse
Background
Doublecortin or DCX seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. Mutations in the gene encoding this protein have been linked to the double cortex syndrome.
Doublecortin is highly expressed in neuronal cells of fetal brain but absent from other tissues. In the adult dentate gyrus it is only expressed in cells contributing to adult neurogenesis.