Synaptic Systems

Cat. No.: 174 103

Amount: 50 µg

Price: $375.00

Cat. No. 174 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 38 to 119 from rat Doc2b (UniProt Id: P70610)
Reactivity	Reacts with: rat (P70610), mouse (P70169). Other species not tested yet.
Specificity	Specific for Doc 2b, no cross-reactivity to Doc 2a and Doc 2c. K.O. validated PubMed: 32347796
Data sheet	174_103.pdf

References for Doc2b - 174 103

Disease-linked mutations in Munc18-1 deplete synaptic Doc2.
Guiberson NGL, Black LS, Haller JE, Brukner A, Abramov D, Ahmad S, Xie YX, Sharma M, Burré J
Brain : a journal of neurology (2024) : . 174 103 WB; tested species: mouse

Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission.
Lipstein N, Chang S, Lin KH, López-Murcia FJ, Neher E, Taschenberger H, Brose N
Neuron (2021) : . 174 103 WB; tested species: mouse

Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2.
Kreutzberger AJB, Kiessling V, Doyle CA, Schenk N, Upchurch CM, Elmer-Dixon M, Ward AE, Preobraschenski J, Hussein SS, Tomaka W, Seelheim P, et al.
eLife (2020) 9: . 174 103 WB; tested species: rat

Single synapse glutamate imaging reveals multiple levels of release mode regulation in mammalian synapses.
Farsi Z, Walde M, Klementowicz AE, Paraskevopoulou F, Woehler A
iScience (2021) 241: 101909. 174 103 ICC; tested species: rat

Loss of Doc2b does not influence transmission at Purkinje cell to deep nuclei synapses under physiological conditions.
Khan MM, Regehr WG
eLife (2020) 9: . 174 103 IHC; KO verified; tested species: mouse

Cat. No.: 174 103

Amount: 50 µg

Price: $375.00

Background

The Doc 2 protein family comprises the three isoforms Doc 2a, b and c. All three proteins are composed of a short N-terminal Munc 13 binding region followed by two C-terminal calcium binding domains (C2A and C2B). Doc 2a and b are predominantly expressed in brain and colocalize with small secretory vesicles whereas Doc 2c is mainly expressed in the heart.
The exact mechanism how the Doc proteins are involved in Ca²⁺ induced exocytosis has yet to be determined.

1 ) Groffen AJ et al., J. Neurochem. (2006)
2 ) Groffen AJ et al., J. Biol. Chem. (2004)
3 ) Korteweg N et al., Eur. J. Neurosci. (2000)
4 ) Duncan RR et al., J. Biol. Chem. (1999)
5 ) Charvin N et al., Biochem. J. (1999)
6 ) Mochida S et al., Proc. Natl. Acad. Sci. U.S.A. (1998)
7 ) Orita S et al., J. Biol. Chem. (1997)
8 ) Verhage M et al., Neuron (1997)
9 ) Kojima T et al., J. Biochem. (1996)
10 ) Orita S et al., Biochem. Biophys. Res. Commun. (1995)

Protocols

Doc2b antibody - 174 103 K.O.

References for Doc2b - 174 103