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DAT antibody - 284 006

DAT is a neurotransmitter transporter involved in the termination of dopamine transmission
Chicken polyclonal purified antibody
Cat. No.: 284 006
Amount: 50 µg
Price: $380.00
Cat. No. 284 006 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500      gallery  
IHC-P: 1 : 500 (see remarks) gallery  
Immunogen Synthetic peptide corresponding to AA 7 to 22 from rat DAT (UniProt Id: P23977)
Reactivity Reacts with: mouse (Q01959), rat (P23977).
Other species not tested yet.
Remarks

IHC-P: Requires heat mediated antigen retrieval with 10 mM Tris pH 9.0, 1 mM EDTA and 0.05 % Tween 20.

Data sheet 284_006.pdf
Cat. No.: 284 006
Amount: 50 µg
Price: $380.00
Background
During neurotransmission neurotransmitters like GABA, glycine, glutamate, dopamine, etc. are released from synaptic vesicles into the synaptic cleft. The dopamine active transporter (DAT) translocates the neurotransmitter dopamine back into the cytoplasm of the neuron.