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DAT antibody - 284 005

DAT is a neurotransmitter transporter involved in the termination of dopamine transmission
Guinea pig polyclonal purified antibody
Cat. No.: 284 005
Amount: 50 µg
Price: $460.00
Cat. No. 284 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: not tested yet
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
EM: yes
Immunogen Synthetic peptide corresponding to AA 7 to 22 from rat DAT (UniProt Id: P23977)
Reactivity Reacts with: rat (P23977), mouse (Q01959).
Other species not tested yet.
Data sheet 284_005.pdf

References for DAT - 284 005

Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson's disease.
Vidyadhara DJ, Somayaji M, Wade N, Yücel B, Zhao H, Shashaank N, Ribaudo J, Gupta J, Lam TT, Sames D, Greene LE, et al.
Cell reports (2023) 423: 112231. 284 005 IHC, EM; tested species: mouse
Molecular interrogation of hypothalamic organization reveals distinct dopamine neuronal subtypes.
Romanov RA, Zeisel A, Bakker J, Girach F, Hellysaz A, Tomer R, Alpár A, Mulder J, Clotman F, Keimpema E, Hsueh B, et al.
Nature neuroscience (2017) 202: 176-188. 284 005 IHC
Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson's disease.
Vidyadhara DJ, Somayaji M, Wade N, Yücel B, Zhao H, Shashaank N, Ribaudo J, Gupta J, Lam TT, Sames D, Greene LE, et al.
Cell reports (2023) 423: 112231. 284 005 IHC, EM; tested species: mouse
Cat. No.: 284 005
Amount: 50 µg
Price: $460.00
Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson's disease.
Vidyadhara DJ, Somayaji M, Wade N, Yücel B, Zhao H, Shashaank N, Ribaudo J, Gupta J, Lam TT, Sames D, Greene LE, et al.
Cell reports (2023) 423: 112231. 284 005 IHC, EM; tested species: mouse
Molecular interrogation of hypothalamic organization reveals distinct dopamine neuronal subtypes.
Romanov RA, Zeisel A, Bakker J, Girach F, Hellysaz A, Tomer R, Alpár A, Mulder J, Clotman F, Keimpema E, Hsueh B, et al.
Nature neuroscience (2017) 202: 176-188. 284 005 IHC
Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson's disease.
Vidyadhara DJ, Somayaji M, Wade N, Yücel B, Zhao H, Shashaank N, Ribaudo J, Gupta J, Lam TT, Sames D, Greene LE, et al.
Cell reports (2023) 423: 112231. 284 005 IHC, EM; tested species: mouse
Background
During neurotransmission neurotransmitters like GABA, glycine, glutamate, dopamine, etc. are released from synaptic vesicles into the synaptic cleft. The dopamine active transporter (DAT) translocates the neurotransmitter dopamine back into the cytoplasm of the neuron.