Synaptic Systems - Cytohesin3

Cat. No.: 212 303

Amount: 50 µg

Price: $375.00

WB

ICC

IHC

Background

Cytohesins are a group of guanine nucleotide exchange factors (GEFs) that contain an N-terminal coil, a central Sec-7 and a C-terminal pleckstrin homology (ph) domain.
They control the activity of ADP-ribosylation factors (Arfs) and are involved in the control of vesicular transport and organization of the actin cytoskeleton.
Cytohesins have also been reported to bind to integrins, a diverse family of heterodimeric transmembrane adhesion receptors that are present on most vertebrate cell types. These proteins are known to be involved in cell-cell or cell-matrix interactions.

1 ) Li CC, Molecular biology of the cell (2007) 1811: 4420-37.
2 ) Dierks H, The Journal of biological chemistry (2001) 27640: 37472-81.
3 ) Ogasawara M, The Journal of biological chemistry (2000) 2755: 3221-30.
4 ) Betz SF, Proceedings of the National Academy of Sciences of the United States of America (1998) 9514: 7909-14.
5 ) Meacci E, Proceedings of the National Academy of Sciences of the United States of America (1997) 945: 1745-8.

Protocols

WB

IP

Standard protocol

ICC

IHC

Cat. No. 212 303	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: not tested yet
Immunogen	Recombinant protein corresponding to AA 1 to 70 from rat Cytohesin3 (UniProt Id: P97696)
Reactivity	Reacts with: rat (P97696), mouse (O08967). Other species not tested yet.
Data sheet	212_303.pdf

Cytohesin3 antibody - 212 303