Cytohesin3 antibody - 212 303

Cytohesins are a group of guanine nucleotide exchange factors (GEFs)

Rabbit polyclonal purified antibody

Cat. No.: 212 303

Amount: 50 µg

Price: $375.00

Cat. No. 212 303	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 70 from rat Cytohesin3 (UniProt Id: P97696)
Reactivity	Reacts with: rat (P97696), mouse (O08967). Other species not tested yet.
Data sheet	212_303.pdf

Cat. No.: 212 303

Amount: 50 µg

Price: $375.00

ICC

IHC

Background

Cytohesins are a group of guanine nucleotide exchange factors (GEFs) that contain an N-terminal coil, a central Sec-7 and a C-terminal pleckstrin homology (ph) domain.
They control the activity of ADP-ribosylation factors (Arfs) and are involved in the control of vesicular transport and organization of the actin cytoskeleton.
Cytohesins have also been reported to bind to integrins, a diverse family of heterodimeric transmembrane adhesion receptors that are present on most vertebrate cell types. These proteins are known to be involved in cell-cell or cell-matrix interactions.

1 ) Li CC et al., Mol. Biol. Cell (2007)
2 ) Dierks H et al., J. Biol. Chem. (2001)
3 ) Ogasawara M et al., J. Biol. Chem. (2000)
4 ) Betz SF et al., Proc. Natl. Acad. Sci. U.S.A. (1998)
5 ) Meacci E et al., Proc. Natl. Acad. Sci. U.S.A. (1997)

Protocols

Standard protocol

ICC

IHC