Cytohesin1/2/3/4 antibody - 212 003

Cytohesins are a group of guanine nucleotide exchange factors (GEFs)

Rabbit polyclonal purified antibody

Cat. No.: 212 003

Amount: 50 µg

Price: $380.00

Cat. No. 212 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 260 to 379 from mouse Cytohesin1 (UniProt Id: Q9QX11)
Reactivity	Reacts with: human (Q15438, Q99418, O43739, Q9UIA0), rat (P97694, P63035, P97696, D3ZHR6), mouse (Q9QX11, P63034, O08967, Q80YW0). Other species not tested yet.
Specificity	Recognizes cytohesin 1, 2, 3, and 4.
Data sheet	212_003.pdf

Cat. No.: 212 003

Amount: 50 µg

Price: $380.00

Background

Cytohesins are a group of guanine nucleotide exchange factors (GEFs) that contain an N-terminal coil, a central Sec-7 and a C-terminal pleckstrin homology (ph) domain.
They control the activity of ADP-ribosylation factors (Arfs) and are involved in the control of vesicular transport and organization of the actin cytoskeleton.
Cytohesins have also been reported to bind to integrins, a diverse family of heterodimeric transmembrane adhesion receptors that are present on most vertebrate cell types. These proteins are known to be involved in cell-cell or cell-matrix interactions.

1 ) Li CC et al., Mol. Biol. Cell (2007)
2 ) Dierks H et al., J. Biol. Chem. (2001)
3 ) Ogasawara M et al., J. Biol. Chem. (2000)
4 ) Betz SF et al., Proc. Natl. Acad. Sci. U.S.A. (1998)
5 ) Meacci E et al., Proc. Natl. Acad. Sci. U.S.A. (1997)

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