Synaptic Systems - Cytohesin1/2/3/4

Cat. No.: 212 003

Amount: 50 µg

Price: $375.00

WB

Background

Cytohesins are a group of guanine nucleotide exchange factors (GEFs) that contain an N-terminal coil, a central Sec-7 and a C-terminal pleckstrin homology (ph) domain.
They control the activity of ADP-ribosylation factors (Arfs) and are involved in the control of vesicular transport and organization of the actin cytoskeleton.
Cytohesins have also been reported to bind to integrins, a diverse family of heterodimeric transmembrane adhesion receptors that are present on most vertebrate cell types. These proteins are known to be involved in cell-cell or cell-matrix interactions.

1 ) Li CC, Molecular biology of the cell (2007) 1811: 4420-37.
2 ) Dierks H, The Journal of biological chemistry (2001) 27640: 37472-81.
3 ) Ogasawara M, The Journal of biological chemistry (2000) 2755: 3221-30.
4 ) Betz SF, Proceedings of the National Academy of Sciences of the United States of America (1998) 9514: 7909-14.
5 ) Meacci E, Proceedings of the National Academy of Sciences of the United States of America (1997) 945: 1745-8.

Protocols

WB

Cat. No. 212 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet
Immunogen	Recombinant protein corresponding to AA 260 to 379 from mouse Cytohesin1 (UniProt Id: Q9QX11)
Reactivity	Reacts with: human (Q15438, Q99418, O43739, Q9UIA0), rat (P97694, P63035, P97696, D3ZHR6), mouse (Q9QX11, P63034, O08967, Q80YW0). Other species not tested yet.
Specificity	Recognizes cytohesin 1, 2, 3, and 4.
Data sheet	212_003.pdf

Cytohesin1/2/3/4 antibody - 212 003