Synaptic Systems

Cat. No.: 325 005

Amount: 50 µg

Price: $470.00

Cat. No. 325 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 541 to 812 from rat Ctip2 (UniProt Id: H9N1L3)
Reactivity	Reacts with: rat (H9N1L3), mouse (Q99PV8). Other species not tested yet.
Data sheet	325_005.pdf

References for Ctip2 - 325 005

Retrosplenial inputs drive visual representations in the medial entorhinal cortex.
Dubanet O, Higley MJ
Cell reports (2024) 437: 114470. 325 005 IHC; tested species: mouse

Acetylcholine synergizes with netrin-1 to drive persistent firing in the entorhinal cortex.
Glasgow SD, Fisher TAJ, Wong EW, Lançon K, Feighan KM, Beamish IV, Gibon J, Séguéla P, Ruthazer ES, Kennedy TE
Cell reports (2024) 432: 113812. 325 005 IHC; tested species: mouse

CaMKIIα Expressing Neurons to Report Activity-Related Endogenous Hypoxia upon Motor-Cognitive Challenge.
Butt UJ, Hassouna I, Fernandez Garcia-Agudo L, Steixner-Kumar AA, Depp C, Barnkothe N, Zillmann MR, Ronnenberg A, Bonet V, Goebbels S, Nave KA, et al.
International journal of molecular sciences (2021) 226: . 325 005 IHC; tested species: mouse

Brain erythropoietin fine-tunes a counterbalance between neurodifferentiation and microglia in the adult hippocampus.
Fernandez Garcia-Agudo L, Steixner-Kumar AA, Curto Y, Barnkothe N, Hassouna I, Jähne S, Butt UJ, Grewe K, Weber MS, Green K, Rizzoli S, et al.
Cell reports (2021) 368: 109548. 325 005 IHC; tested species: mouse

Functional hypoxia drives neuroplasticity and neurogenesis via brain erythropoietin.
Wakhloo D, Scharkowski F, Curto Y, Javed Butt U, Bansal V, Steixner-Kumar AA, Wüstefeld L, Rajput A, Arinrad S, Zillmann MR, Seelbach A, et al.
Nature communications (2020) 111: 1313. 325 005 IHC; tested species: mouse

Revisiting adult neurogenesis and the role of erythropoietin for neuronal and oligodendroglial differentiation in the hippocampus.
Hassouna I, Ott C, Wüstefeld L, Offen N, Neher RA, Mitkovski M, Winkler D, Sperling S, Fries L, Goebbels S, Vreja IC, et al.
Molecular psychiatry (2016) 2112: 1752-1767. 325 005 IHC

Cat. No.: 325 005

Amount: 50 µg

Price: $470.00

Retrosplenial inputs drive visual representations in the medial entorhinal cortex.
Dubanet O, Higley MJ
Cell reports (2024) 437: 114470. 325 005 IHC; tested species: mouse

ICC

IHC

IHC-P

Background

The Coup-TFI interacting protein 2 (Ctip 2), also referred to as Bcl 11b, is a zinc finger transcription factor that is expressed in postmitotic neurons.
Together with Satb 2, Coup-TFI, and Fezf2 it is involved in the fine tuned sequential formation and specification of the different excitatory neuron populations forming the definitive six-layered cortical structure.

1 ) Srivatsa S et al., Nat Commun (2014)
2 ) Adiningrat A et al., J. Med. Invest. (2014)
3 ) Cherrier T et al., Proc. Natl. Acad. Sci. U.S.A. (2013)
4 ) Díaz-Alonso J et al., J. Neurosci. (2012)
5 ) Simon R et al., EMBO J. (2012)
6 ) Chen B et al., Proc. Natl. Acad. Sci. U.S.A. (2008)
7 ) Leid M et al., Gene Expr. Patterns (2004)

Protocols

ICC

IHC

IHC-P

Other products for this target

Ctip2 antibody C-terminus - 325 005

References for Ctip2 - 325 005