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Home > Products > 193 003

CtBP2 antibody - 193 003 K.D.

CtBP1 is a transcriptional corepressor
Rabbit polyclonal purified antibody
Cat. No.: 193 003
Amount: 50 µg
Price: $380.00
Cat. No. 193 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 10000 (see remarks) gallery  
IHC-P: yes
EM: yes

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen Synthetic peptide corresponding to AA 974 to 988 from rat Ribeye (UniProt Id: Q9EQH5-2)
Reactivity Reacts with: human (P56545), rat (Q9EQH5), mouse (P56546), monkey, cow.
Other species not tested yet.
Specificity This antibody recognizes CtBP 2 and ribeye (cat. no. 192 003). K.D. validated PubMed: 28855251
Remarks

The immunogen is identical to that of anti-ribeye (cat. no. 192 003).
IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 is recommended.
Data sheet 193_003.pdf

References for CtBP2 - 193 003

PICK1 regulates AMPA receptor endocytosis via direct interactions with AP2 α-appendage and dynamin.
Fiuza M, Rostosky CM, Parkinson GT, Bygrave AM, Halemani N, Baptista M, Milosevic I, Hanley JG
The Journal of cell biology (2017) 21610: 3323-3338. 193 003 WB; KD verified; tested species: rat
Differential spatial expression and subcellular localization of CtBP family members in rodent brain.
Hübler D, Rankovic M, Richter K, Lazarevic V, Altrock WD, Fischer KD, Gundelfinger ED, Fejtova A
PloS one (2012) 76: e39710. 193 003 WB, ICC, EM; tested species: mouse,rat
Differential spatial expression and subcellular localization of CtBP family members in rodent brain.
Hübler D, Rankovic M, Richter K, Lazarevic V, Altrock WD, Fischer KD, Gundelfinger ED, Fejtova A
PloS one (2012) 76: e39710. 193 003 WB, ICC, EM; tested species: mouse,rat
Immunohistochemical characterization of bipolar cells in four distantly related avian species.
Balaji V, Haverkamp S, Seth PK, Günther A, Mendoza E, Schmidt J, Herrmann M, Pfeiffer LL, Němec P, Scharff C, Mouritsen H, et al.
The Journal of comparative neurology (2023) 5314: 561-581. 193 003 IHC
Lycium Barbarum Polysaccharides Protect Retina in rd1 Mice During Photoreceptor Degeneration.
Liu F, Zhang J, Xiang Z, Xu D, So KF, Vardi N, Xu Y
Investigative ophthalmology & visual science (2018) 591: 597-611. 193 003 IHC; tested species: mouse
Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors.
Puller C, Arbogast P, Keeley PW, Reese BE, Haverkamp S
PloS one (2017) 123: e0173455. 193 003 IHC; tested species: mouse
Cell type-specific bipolar cell input to ganglion cells in the mouse retina.
Neumann S, Hüser L, Ondreka K, Auler N, Haverkamp S
Neuroscience (2016) 316: 420-32. 193 003 IHC
Morphological and physiological analysis of type-5 and other bipolar cells in the Mouse Retina.
Hellmer CB, Zhou Y, Fyk-Kolodziej B, Hu Z, Ichinose T
Neuroscience (2016) 315: 246-58. 193 003 IHC
Frizzled3 Shapes the Development of Retinal Rod Bipolar Cells.
Shen N, Qu Y, Yu Y, So KF, Goffinet AM, Vardi N, Xu Y, Zhou L
Investigative ophthalmology & visual science (2016) 576: 2788-96. 193 003 IHC; tested species: mouse
Morphology and connectivity of the small bistratified A8 amacrine cell in the mouse retina.
Lee SC, Meyer A, Schubert T, Hüser L, Dedek K, Haverkamp S
The Journal of comparative neurology (2015) 52310: 1529-47. 193 003 IHC
Cone bipolar cells in the retina of the microbat Carollia perspicillata.
Butz E, Peichl L, Müller B
The Journal of comparative neurology (2015) 5236: 963-81. 193 003 IHC
Localization of diacylglycerol lipase alpha and monoacylglycerol lipase during postnatal development of the rat retina.
Cécyre B, Monette M, Beudjekian L, Casanova C, Bouchard JF
Frontiers in neuroanatomy (2014) 8: 150. 193 003 IHC; tested species: rat
The rod pathway of the microbat retina has bistratified rod bipolar cells and tristratified AII amacrine cells.
Müller B, Butz E, Peichl L, Haverkamp S
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 333: 1014-23. 193 003 IHC
Characterization of small-field bistratified amacrine cells in macaque retina labeled by antibodies against synaptotagmin-2.
Neumann S, Haverkamp S
The Journal of comparative neurology (2013) 5213: 709-24. 193 003 IHC; tested species: monkey
Detailed functional and structural characterization of a macular lesion in a rhesus macaque.
Dominik Fischer M, Zobor D, Keliris GA, Shao Y, Seeliger MW, Haverkamp S, Jägle H, Logothetis NK, Smirnakis SM
Documenta ophthalmologica. Advances in ophthalmology (2012) 1253: 179-94. 193 003 IHC
Differential spatial expression and subcellular localization of CtBP family members in rodent brain.
Hübler D, Rankovic M, Richter K, Lazarevic V, Altrock WD, Fischer KD, Gundelfinger ED, Fejtova A
PloS one (2012) 76: e39710. 193 003 WB, ICC, EM; tested species: mouse,rat
Cat. No.: 193 003
Amount: 50 µg
Price: $380.00
PICK1 regulates AMPA receptor endocytosis via direct interactions with AP2 α-appendage and dynamin.
Fiuza M, Rostosky CM, Parkinson GT, Bygrave AM, Halemani N, Baptista M, Milosevic I, Hanley JG
The Journal of cell biology (2017) 21610: 3323-3338. 193 003 WB; KD verified; tested species: rat
Differential spatial expression and subcellular localization of CtBP family members in rodent brain.
Hübler D, Rankovic M, Richter K, Lazarevic V, Altrock WD, Fischer KD, Gundelfinger ED, Fejtova A
PloS one (2012) 76: e39710. 193 003 WB, ICC, EM; tested species: mouse,rat
Differential spatial expression and subcellular localization of CtBP family members in rodent brain.
Hübler D, Rankovic M, Richter K, Lazarevic V, Altrock WD, Fischer KD, Gundelfinger ED, Fejtova A
PloS one (2012) 76: e39710. 193 003 WB, ICC, EM; tested species: mouse,rat
Immunohistochemical characterization of bipolar cells in four distantly related avian species.
Balaji V, Haverkamp S, Seth PK, Günther A, Mendoza E, Schmidt J, Herrmann M, Pfeiffer LL, Němec P, Scharff C, Mouritsen H, et al.
The Journal of comparative neurology (2023) 5314: 561-581. 193 003 IHC
Lycium Barbarum Polysaccharides Protect Retina in rd1 Mice During Photoreceptor Degeneration.
Liu F, Zhang J, Xiang Z, Xu D, So KF, Vardi N, Xu Y
Investigative ophthalmology & visual science (2018) 591: 597-611. 193 003 IHC; tested species: mouse
Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors.
Puller C, Arbogast P, Keeley PW, Reese BE, Haverkamp S
PloS one (2017) 123: e0173455. 193 003 IHC; tested species: mouse
Cell type-specific bipolar cell input to ganglion cells in the mouse retina.
Neumann S, Hüser L, Ondreka K, Auler N, Haverkamp S
Neuroscience (2016) 316: 420-32. 193 003 IHC
Morphological and physiological analysis of type-5 and other bipolar cells in the Mouse Retina.
Hellmer CB, Zhou Y, Fyk-Kolodziej B, Hu Z, Ichinose T
Neuroscience (2016) 315: 246-58. 193 003 IHC
Frizzled3 Shapes the Development of Retinal Rod Bipolar Cells.
Shen N, Qu Y, Yu Y, So KF, Goffinet AM, Vardi N, Xu Y, Zhou L
Investigative ophthalmology & visual science (2016) 576: 2788-96. 193 003 IHC; tested species: mouse
Morphology and connectivity of the small bistratified A8 amacrine cell in the mouse retina.
Lee SC, Meyer A, Schubert T, Hüser L, Dedek K, Haverkamp S
The Journal of comparative neurology (2015) 52310: 1529-47. 193 003 IHC
Cone bipolar cells in the retina of the microbat Carollia perspicillata.
Butz E, Peichl L, Müller B
The Journal of comparative neurology (2015) 5236: 963-81. 193 003 IHC
Localization of diacylglycerol lipase alpha and monoacylglycerol lipase during postnatal development of the rat retina.
Cécyre B, Monette M, Beudjekian L, Casanova C, Bouchard JF
Frontiers in neuroanatomy (2014) 8: 150. 193 003 IHC; tested species: rat
The rod pathway of the microbat retina has bistratified rod bipolar cells and tristratified AII amacrine cells.
Müller B, Butz E, Peichl L, Haverkamp S
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 333: 1014-23. 193 003 IHC
Characterization of small-field bistratified amacrine cells in macaque retina labeled by antibodies against synaptotagmin-2.
Neumann S, Haverkamp S
The Journal of comparative neurology (2013) 5213: 709-24. 193 003 IHC; tested species: monkey
Detailed functional and structural characterization of a macular lesion in a rhesus macaque.
Dominik Fischer M, Zobor D, Keliris GA, Shao Y, Seeliger MW, Haverkamp S, Jägle H, Logothetis NK, Smirnakis SM
Documenta ophthalmologica. Advances in ophthalmology (2012) 1253: 179-94. 193 003 IHC
Differential spatial expression and subcellular localization of CtBP family members in rodent brain.
Hübler D, Rankovic M, Richter K, Lazarevic V, Altrock WD, Fischer KD, Gundelfinger ED, Fejtova A
PloS one (2012) 76: e39710. 193 003 WB, ICC, EM; tested species: mouse,rat
WB
IHC
Background

CtBP 2 and its close relative CtBP 1 interact with various transcription factors through a PLDLSL motif enhancing transcriptional repression. With the exception of the first 20 amino acids CtBP 2 is identical to the B-domain of ribeye, a scaffolding protein of the ribbon synapse. Both proteins originate from the same gene.