Synaptic Systems

Cat. No.: 154 003

Amount: 50 µg

Price: $380.00

Cat. No. 154 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery IP: yes ICC: 1 : 100 up to 1 : 1000 gallery IHC: yes gallery IHC-P: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 182 to 198 from rat CSP (UniProt Id: P60905)
Reactivity	Reacts with: human (Q9H3Z4), rat (P60905), mouse (P60904), cow, dog, chicken. Other species not tested yet.
Specificity	K.O. validated PubMed: 27881461
Data sheet	154_003.pdf

References for CSP - 154 003

LuTHy: a double-readout bioluminescence-based two-hybrid technology for quantitative mapping of protein-protein interactions in mammalian cells.
Trepte P, Kruse S, Kostova S, Hoffmann S, Buntru A, Tempelmeier A, Secker C, Diez L, Schulz A, Klockmeier K, Zenkner M, et al.
Molecular systems biology (2018) 147: e8071. 154 003 WB, ICC; tested species: mouse

Gestational exposure to metformin programs improved glucose tolerance and insulin secretion in adult male mouse offspring.
Gregg BE, Botezatu N, Brill JD, Hafner H, Vadrevu S, Satin LS, Alejandro EU, Bernal-Mizrachi E
Scientific reports (2018) 81: 5745. 154 003 WB; tested species: mouse

Lysosomal dysfunction disrupts presynaptic maintenance and restoration of presynaptic function prevents neurodegeneration in lysosomal storage diseases.
Sambri I, D'Alessio R, Ezhova Y, Giuliano T, Sorrentino NC, Cacace V, De Risi M, Cataldi M, Annunziato L, De Leonibus E, Fraldi A, et al.
EMBO molecular medicine (2017) 91: 112-132. 154 003 WB, ICC; KO verified; tested species: mouse

Ubiquitin-Synaptobrevin Fusion Protein Causes Degeneration of Presynaptic Motor Terminals in Mice.
Liu Y, Li H, Sugiura Y, Han W, Gallardo G, Khvotchev M, Zhang Y, Kavalali ET, Südhof TC, Lin W
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3533: 11514-31. 154 003 WB

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 154 003 WB, ICC, IHC; tested species: mouse,rat

Molecular dynamics of photoreceptor synapse formation in the developing chick retina.
Wahlin KJ, Moreira EF, Huang H, Yu N, Adler R
The Journal of comparative neurology (2008) 5065: 822-37. 154 003 WB, IHC

Cysteine string protein-alpha prevents activity-dependent degeneration in GABAergic synapses.
García-Junco-Clemente P, Cantero G, Gómez-Sánchez L, Linares-Clemente P, Martínez-López JA, Luján R, Fernández-Chacón R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3021: 7377-91. 154 003 ICC

Selective coexpression of synaptic proteins, α-synuclein, cysteine string protein-α, synaptophysin, synaptotagmin-1, and synaptobrevin-2 in vesicular acetylcholine transporter-immunoreactive axons in the guinea pig ileum.
Sharrad DF, Gai WP, Brookes SJ
The Journal of comparative neurology (2013) 52111: 2523-37. 154 003 IHC

α-Syn suppression reverses synaptic and memory defects in a mouse model of dementia with Lewy bodies.
Lim Y, Kehm VM, Lee EB, Soper JH, Li C, Trojanowski JQ, Lee VM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3127: 10076-87. 154 003 IHC

Cat. No.: 154 003

Amount: 50 µg

Price: $380.00

ICC

IHC

Background

Cysteine String Proteins CSPs are composed of an N-terminal J-domain and a central palmitoylated cysteine string. This post-translational modification shifts the molecular weight of CSP 1 in brain from 23 kDa to 34 kDa and confers membrane targeting of the protein.
CSP has been initially identified as a synaptic vesicle protein which is involved in Ca²⁺ triggered neurotransmitter release. Later CSP was also found on Large Dense Core Vesicles (LDCVs) of pancreatic insulin secretory β-cells, chromaffin cells and adipocytes. It has been shown to interact with SNARE proteins like VAMP 2, VAMP 7 and syntaxin 4.

1 ) Smith GB et al., J. Biol. Chem. (2005)
2 ) Evans GJ et al., Eur. J. Neurosci. (2005)
3 ) Miller LC et al., J. Biol. Chem. (2003)
4 ) Chamberlain LH et al., J. Cell. Sci. (2001)
5 ) Chamberlain LH et al., Biochem. J. (1998)
6 ) Chamberlain LH et al., J. Biol. Chem. (1997)
7 ) Chamberlain LH et al., J. Biol. Chem. (1996)

Protocols

Standard protocol

ICC

IHC

IHC-P

Other products for this target

CSP antibody - 154 003 K.O.

References for CSP - 154 003