Synaptic Systems - Cre-Recombinase

Cat. No.: 257 003

Amount: 50 µg

Price: $380.00

Cat. No. 257 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: yes IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Full length recombinant Cre-recombinase from Bacteriophage P1. (UniProt Id: P06956)
Specificity	Recognizes Cre- and iCre-recombinase.
Data sheet	257_003.pdf

References for Cre-Recombinase - 257 003

CYFIP2 p.Arg87Cys Causes Neurological Defects and Degradation of CYFIP2.
Kang M, Zhang Y, Kang HR, Kim S, Ma R, Yi Y, Lee S, Kim Y, Li H, Jin C, Lee D, et al.
Annals of neurology (2023) 931: 155-163. 257 003 WB; tested species: mouse

Context-dependent requirement of G protein coupling for Latrophilin-2 in target selection of hippocampal axons.
Pederick DT, Perry-Hauser NA, Meng H, He Z, Javitch JA, Luo L
eLife (2023) 12: . 257 003 IHC; tested species: mouse

Human OPRM1 and murine Oprm1 promoter driven viral constructs for genetic access to μ-opioidergic cell types.
Salimando GJ, Tremblay S, Kimmey BA, Li J, Rogers SA, Wojick JA, McCall NM, Wooldridge LM, Rodrigues A, Borner T, Gardiner KL, et al.
Nature communications (2023) 141: 5632. 257 003 IHC; tested species: mouse

A critical period of translational control during brain development at codon resolution.
Harnett D, Ambrozkiewicz MC, Zinnall U, Rusanova A, Borisova E, Drescher AN, Couce-Iglesias M, Villamil G, Dannenberg R, Imami K, Münster-Wandowski A, et al.
Nature structural & molecular biology (2022) 2912: 1277-1290. 257 003 IHC; tested species: mouse

The role of α-tubulin tyrosination in controlling the structure and function of hippocampal neurons.
Hosseini S, van Ham M, Erck C, Korte M, Michaelsen-Preusse K
Frontiers in molecular neuroscience (2022) 15: 931859. 257 003 IHC; tested species: mouse

Cerebellin-2 regulates a serotonergic dorsal raphe circuit that controls compulsive behaviors.
Seigneur E, Wang J, Dai J, Polepalli J, Südhof TC
Molecular psychiatry (2021) : . 257 003 IHC; tested species: mouse

Npas1+-Nkx2.1+ Neurons Are an Integral Part of the Cortico-pallido-cortical Loop.
Abecassis ZA, Berceau BL, Win PH, García D, Xenias HS, Cui Q, Pamukcu A, Cherian S, Hernández VM, Chon U, Lim BK, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 404: 743-768. 257 003 IHC; tested species: mouse

Regulation of BDNF Release by ARMS/Kidins220 through Modulation of Synaptotagmin-IV Levels.
López-Benito S, Sánchez-Sánchez J, Brito V, Calvo L, Lisa S, Torres-Valle M, Palko ME, Vicente-García C, Fernández-Fernández S, Bolaños JP, Ginés S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) 3823: 5415-5428. 257 003 IHC; tested species: mouse

Polarity Acquisition in Cortical Neurons Is Driven by Synergistic Action of Sox9-Regulated Wwp1 and Wwp2 E3 Ubiquitin Ligases and Intronic miR-140.
Ambrozkiewicz MC, Schwark M, Kishimoto-Suga M, Borisova E, Hori K, Salazar-Lázaro A, Rusanova A, Altas B, Piepkorn L, Bessa P, Schaub T, et al.
Neuron (2018) : . 257 003 IHC; tested species: mouse

Satb2Cre/+ mouse as a tool to investigate cell fate determination in the developing neocortex.
Ambrozkiewicz MC, Bessa P, Salazar-Lázaro A, Salina V, Tarabykin V
Journal of neuroscience methods (2017) 291: 113-121. 257 003 IHC; tested species: mouse

Cat. No.: 257 003

Amount: 50 µg

Price: $380.00

ICC

IHC

Background

Cre-Recombinase is a 38 kDa type 1 topoisomerase from bacteriophage P1. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA and catalyzes site-specific recombination between two 34-base-pair LOXP recognition sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. Its highly specific catalytic activity makes it a valuable tool for the generation of conditional or tissue specific mutants.

1 ) Ferguson BW et al., PLoS ONE (2012)
2 ) Lanza AM et al., Biotechnol J (2012)
3 ) Smedley D et al., Methods (2011)
4 ) Brault V et al., Handb Exp Pharmacol (2007)
5 ) Abremski K et al., J. Biol. Chem. (1984)
6 ) Hoess R et al., Cold Spring Harb. Symp. Quant. Biol. (1984)

Protocols

ICC

IHC

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Cre-Recombinase antibody - 257 003

References for Cre-Recombinase - 257 003