COX4 antibody - 298 002

COX4 is a protein of the inner mitochondrial membrane

Rabbit polyclonal antiserum

Cat. No.: 298 002

Amount: 200 µl

Price: $355.00

Cat. No. 298 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not recommended ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1 to 18 from mouse COX4 (UniProt Id: P19783)
Reactivity	Reacts with: rat (P10888), mouse (P13073). Other species not tested yet.
Matching control protein/peptide	298-0P
Data sheet	298_002.pdf

References for COX4 - 298 002

Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity.
Yagensky O, Kohansal-Nodehi M, Gunaseelan S, Rabe T, Zafar S, Zerr I, Härtig W, Urlaub H, Chua JJ
eLife (2019) 8: . 298 002 WB; tested species: mouse

Cat. No.: 298 002

Amount: 200 µl

Price: $355.00

ICC

IHC

IHC-P

Background

The enzyme cytochrome C oxidase or Complex 4 is a large heterooligomeric enzyme consisting of 13 subunits. It is localized to the inner mitochondrial membrane. It catalyzes the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane. The resulting proton gradient finaly drives ATP synthesis.

1 ) Böttinger L et al., Mol. Biol. Cell (2013)
2 ) Su X et al., Mol. Cell. Biol. (2006)
3 ) Ostermeier C et al., Curr. Opin. Struct. Biol. (1996)

Protocols

ICC

IHC

IHC-P

OTHER

Preabsorbtion

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