Cat. No.: 313 111
Amount: 100 µg
Price:
$415.00
Cat. No. 313 111 |
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: yes (see remarks) gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: not tested yet Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.', $event)" style="cursor: help;">ELISA: yes |
Clone | 289H10 |
Subtype | IgG1 (κ light chain) |
Immunogen | Recombinant protein corresponding to AA 1 to 513 from human Cortactin (UniProt Id: Q14247) |
Epitop |
AA 147 to 509 from human Cortactin (UniProt Id: Q14247) |
Reactivity |
Reacts with: human (Q14247), mouse (Q60598). Other species not tested yet. |
Remarks |
ICC: Works on PFA and methanol fixed cells, but methanol fixation produces less background. |
Data sheet | 313_111.pdf |
Cortactin is a cortical actin binding protein and the major substrate for the tyrosine kinase v-Src. The interaction of its N-terminal acidic domain (NTA) with the actin nucleation factor Arp 2/3 links cytoskeletal organization with signal transduction. The carboxy terminus comprises a proline rich region and an SH3 domain that can interact with several scaffolding proteins like CortBP1 and Shank 3. In neurons cortactin is concentrated in dendritic spines and involved in spine targeting.