Cat. No.: 122 402
Amount: 200 µl
Price:
$355.00
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| Cat. No. 122 402 |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Immunogen | Recombinant protein corresponding to AA 1 to 160 from mouse Complexin4 (UniProt Id: Q80WM3) |
| Reactivity |
Reacts with: rat (D3ZM85), mouse (Q80WM3). Other species not tested yet. |
| Specificity | Specific for complexin 4, no cross reaction to other complexins. K.O. validated PubMed: 19386896 |
| Remarks |
IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 is recommended. |
| Data sheet | 122_402.pdf |
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Complexins are enriched in neurons where they colocalize with syntaxin 1 and SNAP 25. In addition, complexin 2 is expressed ubiquitously at low levels. Complexins bind weakly to syntaxin 1 alone and not at all to synaptobrevin and SNAP 25, but strongly to the SNAP receptor-core complex composed of these three molecules. They compete with α-SNAP for binding to the core complex but not with other interacting molecules, suggesting that complexins regulate the sequential interactions of α-SNAP and synaptotagmins with the SNAP receptor during exocytosis.
In retinal ribbon synapses complexin 3 and complexin 4 functionally replace complexin 1 and 2. They have similar biochemical binding properties and are farnesylated at their C-terminus.