Cat. No.: 122 311
Amount: 100 µg
Price:
$415.00
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| Cat. No. 122 311 |
100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Clone | 294C2 |
| Subtype | IgG1 (κ light chain) |
| Immunogen | Recombinant protein corresponding to AA 1 to 158 from mouse Complexin3 (UniProt Id: Q8R1B5) |
| Reactivity |
Reacts with: rat (D4ABY0), mouse (Q8R1B5). Other species not tested yet. |
| Specificity | Specific for complexin 3, no cross reaction to other complexins. K.O. validated |
| Remarks |
IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 is recommended. |
| Data sheet | 122_311.pdf |
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Complexins are enriched in neurons where they colocalize with syntaxin 1 and SNAP 25. In addition, complexin 2 is expressed ubiquitously at low levels. Complexins bind weakly to syntaxin 1 alone and not at all to synaptobrevin and SNAP 25, but strongly to the SNAP receptor-core complex composed of these three molecules. They compete with α-SNAP for binding to the core complex but not with other interacting molecules, suggesting that complexins regulate the sequential interactions of α-SNAP and synaptotagmins with the SNAP receptor during exocytosis.
In retinal ribbon synapses complexin 3 and complexin 4 functionally replace complexin 1 and 2. They have similar biochemical binding properties and are farnesylated at their C-terminus.