Cat. No.: 261 111
Amount: 100 µg
Price:
$415.00
Cat. No. 261 111 |
100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: yes Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: not tested yet Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not recommended |
Clone | 197A10 |
Subtype | IgG2b (κ light chain) |
Immunogen | Recombinant protein corresponding to AA 1 to 471 from rat Collybistin (UniProt Id: Q9QX73) |
Epitop |
AA 4 to 229 from rat Collybistin (UniProt Id: Q9QX73) |
Reactivity |
Reacts with: rat (Q9QX73), mouse (Q3UTH8). Other species not tested yet. |
Specificity | Specific for collybistin, immunogen present in all three described splice-variants. |
Data sheet | 261_111.pdf |
The GDP/GTP-exchange factor collybistin is composed of a dbl homology domain (DH) and a pleckstrin homology domain (PH) connected by a linker sequence. Three splice variants with different C-terminal regions have been described, so far.
Collybistin is supposed to be involved in the clustering of gephyrin, a scaffolding protein linking glycine and GABA receptors to microtubuli.