CNP1 antibody - 355 003 K.O.

CNP1 is a marker for oligodendrocytes

Rabbit polyclonal purified antibody

Cat. No.: 355 003

Amount: 50 µg

Price: $455.00

Cat. No. 355 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 420 from mouse CNP1 (UniProt Id: P16330)
Reactivity	Reacts with: rat (P13233), mouse (P16330). Other species not tested yet.
Specificity	K.O. validated
Data sheet	355_003.pdf

References for CNP1 - 355 003

Development and Optimization of a Multilayer Rat Purkinje Neuron Culture.
Uggerud IM, Kråkenes T, Hirai H, Vedeler CA, Schubert M
Cerebellum (London, England) (2023) : . 355 003 ICC; tested species: rat

Characterization of dUTPase expression in mouse postnatal development and adult neurogenesis.
Nagy N, Hádinger N, Tóth O, Rácz GA, Pintér T, Gál Z, Urbán M, Gócza E, Hiripi L, Acsády L, Vértessy BG, et al.
Scientific reports (2024) 141: 13139. 355 003 IHC; tested species: mouse

Cat. No.: 355 003

Amount: 50 µg

Price: $455.00

ICC

IHC

IHC-P

Background

The 2', 3'-cyclic nucleotide 3'-phosphodiesterase CNP 1, also referred to as CNPase and CNP, is one of the most abundant membrane-associated enzymes in the myelin sheath of the vertebrate nervous system.
It is assumed that CNP 1 participates in RNA metabolism of myelinating oligodendrocytes.

1 ) Myllykoski M et al., PLoS ONE (2012)
2 ) Edgar NM et al., Transl Psychiatry (2011)
3 ) Lappe-Siefke C et al., Nat. Genet. (2003)
4 ) Brunner C et al., J. Neurochem. (1989)
5 ) Braun PE et al., J. Neurosci. (1988)

Protocols

Standard protocol

ICC

IHC

IHC-P

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