CNIH2 antibody - 253 203

CNIH2 is a GluA interacting protein

Rabbit polyclonal purified antibody

Cat. No.: 253 203

Amount: 50 µg

Price: $375.00

Cat. No. 253 203	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 107 to 121 from mouse CNIH2 (UniProt Id: O35089)
Reactivity	Reacts with: rat (Q5BJU5), mouse (O35089). Other species not tested yet.
Matching control protein/peptide	253-2P
Data sheet	253_203.pdf

References for CNIH2 - 253 203

Deletion of olfactomedin 2 induces changes in the AMPA receptor complex and impairs visual, olfactory, and motor functions in mice.
Sultana A, Nakaya N, Dong L, Abu-Asab M, Qian H, Tomarev SI
Experimental neurology (2014) 261: 802-11. 253 203 WB

Cat. No.: 253 203

Amount: 50 µg

Price: $375.00

Background

Ligand gated cation channels of the AMPA/GLuA subtype interact with a family of proteins termed transmembrane AMPA-receptor regulatory proteins (TARPs). These proteins regulate the surface expression and the biophysical properties of GluAs.
A second protein family of 4 members referred to as cornichon 1-4 or CNIH 1-4 has been attributed similar properties in mammals. However it is still under discussion whether these proteins serve as chaperones, involved in the trafficking of EGFR ligands from the ER to Golgi like their homologes in yeast and Drosophila, or as GluA auxilliary subunits. For CNIH 3 three isoforms have been described.

1 ) Gill MB et al., J. Neurosci. (2011)
2 ) Kato AS et al., Neuron (2010)
3 ) Shi Y et al., Proc. Natl. Acad. Sci. U.S.A. (2010)
4 ) Tomita S et al., Physiology (Bethesda) (2010)

Protocols

Standard protocol

OTHER

Preabsorbtion

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