CNGA3 antibody - 267 003 K.O.

CNGA3 is a cyclic nucleotide-gated channel

Rabbit polyclonal purified antibody

Cat. No.: 267 003

Amount: 50 µg

Price: $375.00

Cat. No. 267 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 5000 (see remarks) gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 653 to 670 from rat CNGA3 (UniProt Id: Q9ER33)
Reactivity	Reacts with: rat (Q9ER33), mouse (Q9JJZ8). Other species not tested yet.
Specificity	K.O. validated
Remarks	IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 is recommended.
Data sheet	267_003.pdf

Cat. No.: 267 003

Amount: 50 µg

Price: $375.00

IHC

Background

In the mammalian retina two types of photoreceptors, rods and cones, occur. Their response to light is mediated by the closure of a cyclic nucleotide-gated (CNG) cation channel in the plasma membrane, causing hyperpolarization and a decrease of glutamate release.
In rods the CNG channel is formed by the subunits CNGA1 and CNGB1 and in cones by CNGA3 and CNGB3. Accordingly CNGA3 knockout mice lack any cone-mediated photoresponse.
This antibody is a useful marker for photoreceptors.

1 ) Haverkamp S et al., J. Neurosci. (2006)
2 ) Claes E et al., Invest. Ophthalmol. Vis. Sci. (2004)
3 ) Biel M et al., Proc. Natl. Acad. Sci. U.S.A. (1999)

Protocols

IHC