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ClC3 antibody - 252 003

CLC3 is a chloride channel providing charge balance in acidic organelles
Rabbit polyclonal purified antibody
Cat. No.: 252 003
Amount: 50 µg
Price: $375.00
Cat. No. 252 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 616 to 747 from mouse ClC3 (UniProt Id: P51791)
Reactivity Reacts with: rat (P51792), mouse (P51791).
Other species not tested yet.
Specificity Epitope present in both splice variants of ClC 3. May cross react to ClC 4 and 5, due to sequence homology.
Matching control protein/peptide 252-0P
Data sheet 252_003.pdf

References for ClC3 - 252 003

CAPS1 effects on intragranular pH and regulation of BDNF release from secretory granules in hippocampal neurons.
Eckenstaler R, Lessmann V, Brigadski T
Journal of cell science (2016) 1297: 1378-90. 252 003 ICC; tested species: rat
Cat. No.: 252 003
Amount: 50 µg
Price: $375.00
CAPS1 effects on intragranular pH and regulation of BDNF release from secretory granules in hippocampal neurons.
Eckenstaler R, Lessmann V, Brigadski T
Journal of cell science (2016) 1297: 1378-90. 252 003 ICC; tested species: rat
Background

The ClC 3 chloride channel, also referred to as ClCN 3, belongs to the voltage-gated chloride channels and is ubiquitously expressed throughout the brain and other tissues. Two splice variants of the protein have been described. ClC 3 resides on intracellular trafficking vesicles including synaptic vesicles. It mediates the exchange of chloride ions against protons. In contrast to the translocation of glutamate into synaptic vesicles, the VGAT mediated transport of GABA requires ClC 3. It has been postulated that ClC 3 provides chloride ions as charge balance during the acidification of the vesicle lumen by proton pumping of the V-ATPase.