Synaptic Systems - Claudin11

Cat. No.: 241 003

Amount: 50 µg

Price: $375.00

Immunosignals of Oligodendrocyte Markers and Myelin-Associated Proteins Are Critically Affected after Experimental Stroke in Wild-Type and Alzheimer Modeling Mice of Different Ages.
Michalski D, Keck AL, Grosche J, Martens H, Härtig W
Frontiers in cellular neuroscience (2018) 12: 23. 241 003 IHC; tested species: mouse

WB

ICC

IHC

IHC-P

Background

Claudin 11, also known as Oligodendrocyte specific protein (OSP), is one of the most abundant components of CNS myelin where it locates to the interlamellar strands in myelin sheaths. To date more than 20 members of the claudin family have been identified. They are expressed in epithelial and endothelial cells in all tight junction bearing tissues. Claudins share a common transmembrane topology with 4 transmembrane spanning domains and two extracellular loops.

1 ) Devaux J et al., J. Cell Biol. (2008)
2 ) Kitajiri S et al., J. Cell. Sci. (2004)
3 ) Tiwari-Woodruff SK et al., J. Cell Biol. (2001)
4 ) Bronstein JM et al., J. Neurosci. Res. (2000)
5 ) Morita K et al., J. Cell Biol. (1999)
6 ) Bronstein JM et al., J. Neurosci. Res. (1997)

Protocols

WB

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Cat. No. 241 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 200 up to 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 188 to 207 from rat Claudin11 (UniProt Id: Q99P82)
Reactivity	Reacts with: human (O75508), rat (Q99P82), mouse (Q60771), cow, pig. Other species not tested yet.
Matching control protein/peptide	241-0P
Remarks	WB: Moderate heating (50°C) of samples prior SDS-PAGE improves signal strength.
Data sheet	241_003.pdf

Claudin11 antibody - 241 003

References for Claudin11 - 241 003