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Home > Products > 113 011

Clathrin light chain antibody - 113 011

Clathrin light chain is a small clathrin coat subunit involved in endocytosis
Mouse monoclonal purified IgG
Cat. No.: 113 011
Amount: 100 µg
Price: $415.00
Cat. No. 113 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 5000 up to 1 : 10000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 2000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 57.4
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 156 to 173 from rat Clathrin light chainB (UniProt Id: P08082)
Reactivity Reacts with: human (P09497), rat (P08082), mouse (Q6IRU5), zebrafish.
Other species not tested yet.
Specificity Specific for both neuronal light chains, does not cross-react with the non-neuronal variants.
Remarks

Suitable for immunogold electron microscopy.
Data sheet 113_011.pdf

References for Clathrin light chain - 113 011

Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson's disease.
Vidyadhara DJ, Somayaji M, Wade N, Yücel B, Zhao H, Shashaank N, Ribaudo J, Gupta J, Lam TT, Sames D, Greene LE, et al.
Cell reports (2023) 423: 112231. 113 011 WB, IHC; tested species: mouse
Vesicular Synaptobrevin/VAMP2 Levels Guarded by AP180 Control Efficient Neurotransmission.
Koo SJ, Kochlamazashvili G, Rost B, Puchkov D, Gimber N, Lehmann M, Tadeus G, Schmoranzer J, Rosenmund C, Haucke V, Maritzen T, et al.
Neuron (2015) 882: 330-44. 113 011 WB; tested species: mouse
Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Grønborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, et al.
The EMBO journal (2014) 332: 157-70. 113 011 WB; tested species: mouse
Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 113 011 WB; tested species: mouse
CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 113 011 WB; tested species: mouse
Role for Reelin in neurotransmitter release.
Hellwig S, Hack I, Kowalski J, Brunne B, Jarowyj J, Unger A, Bock HH, Junghans D, Frotscher M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 317: 2352-60. 113 011 WB
APP/PS1KI bigenic mice develop early synaptic deficits and hippocampus atrophy.
Breyhan H, Wirths O, Duan K, Marcello A, Rettig J, Bayer TA
Acta neuropathologica (2009) 1176: 677-85. 113 011 WB; tested species: mouse
A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis.
Ferguson SM, Brasnjo G, Hayashi M, Wölfel M, Collesi C, Giovedi S, Raimondi A, Gong LW, Ariel P, Paradise S, O'toole E, et al.
Science (New York, N.Y.) (2007) 3165824: 570-4. 113 011 WB, ICC
Stimulation of phosphatidylinositol kinase type I-mediated phosphatidylinositol (4,5)-bisphosphate synthesis by AP-2mu-cargo complexes.
Krauss M, Kukhtina V, Pechstein A, Haucke V
Proceedings of the National Academy of Sciences of the United States of America (2006) 10332: 11934-9. 113 011 WB
The neuronal form of adaptor protein-3 is required for synaptic vesicle formation from endosomes.
Blumstein J, Faundez V, Nakatsu F, Saito T, Ohno H, Kelly RB
The Journal of neuroscience : the official journal of the Society for Neuroscience (2001) 2120: 8034-42. 113 011 WB
Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 113 011 WB, IHC
Nanoscopic dopamine transporter distribution and conformation are inversely regulated by excitatory drive and D2 autoreceptor activity.
Lycas MD, Ejdrup AL, Sørensen AT, Haahr NO, Jørgensen SH, Guthrie DA, Støier JF, Werner C, Newman AH, Sauer M, Herborg F, et al.
Cell reports (2022) 4013: 111431. 113 011 ICC; tested species: rat
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy.
Richter KN, Revelo NH, Seitz KJ, Helm MS, Sarkar D, Saleeb RS, D'Este E, Eberle J, Wagner E, Vogl C, Lazaro DF, et al.
The EMBO journal (2018) 371: 139-159. 113 011 ICC; tested species: mouse
JIP3 localises to exocytic vesicles and focal adhesions in the growth cones of differentiated PC12 cells.
Caswell PT, Dickens M
Molecular and cellular biochemistry (2017) : . 113 011 ICC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 113 011 ICC; tested species: rat
A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis.
Ferguson SM, Brasnjo G, Hayashi M, Wölfel M, Collesi C, Giovedi S, Raimondi A, Gong LW, Ariel P, Paradise S, O'toole E, et al.
Science (New York, N.Y.) (2007) 3165824: 570-4. 113 011 WB, ICC
The first synapse in vision in the aging mouse retina.
Gierke K, Lux UT, Regus-Leidig H, Brandstätter JH
Frontiers in cellular neuroscience (2023) 17: 1291054. 113 011 IHC; tested species: mouse
Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson's disease.
Vidyadhara DJ, Somayaji M, Wade N, Yücel B, Zhao H, Shashaank N, Ribaudo J, Gupta J, Lam TT, Sames D, Greene LE, et al.
Cell reports (2023) 423: 112231. 113 011 WB, IHC; tested species: mouse
Modes and regulation of endocytic membrane retrieval in mouse auditory hair cells.
Neef J, Jung S, Wong AB, Reuter K, Pangrsic T, Chakrabarti R, Kügler S, Lenz C, Nouvian R, Boumil RM, Frankel WN, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 343: 705-16. 113 011 IHC; tested species: mouse
Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 113 011 IHC; tested species: rat
Synaptic pathology, altered gene expression, and degeneration in photoreceptors impacted by drusen.
Johnson PT, Brown MN, Pulliam BC, Anderson DH, Johnson LV
Investigative ophthalmology & visual science (2005) 4612: 4788-95. 113 011 IHC
The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina.
von Kriegstein K, Schmitz F
Cell and tissue research (2003) 3112: 159-73. 113 011 IHC
Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 113 011 WB, IHC
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 113 011 DNA-PAINT; tested species: rat
Cat. No.: 113 011
Amount: 100 µg
Price: $415.00
Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson's disease.
Vidyadhara DJ, Somayaji M, Wade N, Yücel B, Zhao H, Shashaank N, Ribaudo J, Gupta J, Lam TT, Sames D, Greene LE, et al.
Cell reports (2023) 423: 112231. 113 011 WB, IHC; tested species: mouse
Vesicular Synaptobrevin/VAMP2 Levels Guarded by AP180 Control Efficient Neurotransmission.
Koo SJ, Kochlamazashvili G, Rost B, Puchkov D, Gimber N, Lehmann M, Tadeus G, Schmoranzer J, Rosenmund C, Haucke V, Maritzen T, et al.
Neuron (2015) 882: 330-44. 113 011 WB; tested species: mouse
Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Grønborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, et al.
The EMBO journal (2014) 332: 157-70. 113 011 WB; tested species: mouse
Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 113 011 WB; tested species: mouse
CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 113 011 WB; tested species: mouse
Role for Reelin in neurotransmitter release.
Hellwig S, Hack I, Kowalski J, Brunne B, Jarowyj J, Unger A, Bock HH, Junghans D, Frotscher M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 317: 2352-60. 113 011 WB
APP/PS1KI bigenic mice develop early synaptic deficits and hippocampus atrophy.
Breyhan H, Wirths O, Duan K, Marcello A, Rettig J, Bayer TA
Acta neuropathologica (2009) 1176: 677-85. 113 011 WB; tested species: mouse
A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis.
Ferguson SM, Brasnjo G, Hayashi M, Wölfel M, Collesi C, Giovedi S, Raimondi A, Gong LW, Ariel P, Paradise S, O'toole E, et al.
Science (New York, N.Y.) (2007) 3165824: 570-4. 113 011 WB, ICC
Stimulation of phosphatidylinositol kinase type I-mediated phosphatidylinositol (4,5)-bisphosphate synthesis by AP-2mu-cargo complexes.
Krauss M, Kukhtina V, Pechstein A, Haucke V
Proceedings of the National Academy of Sciences of the United States of America (2006) 10332: 11934-9. 113 011 WB
The neuronal form of adaptor protein-3 is required for synaptic vesicle formation from endosomes.
Blumstein J, Faundez V, Nakatsu F, Saito T, Ohno H, Kelly RB
The Journal of neuroscience : the official journal of the Society for Neuroscience (2001) 2120: 8034-42. 113 011 WB
Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 113 011 WB, IHC
Nanoscopic dopamine transporter distribution and conformation are inversely regulated by excitatory drive and D2 autoreceptor activity.
Lycas MD, Ejdrup AL, Sørensen AT, Haahr NO, Jørgensen SH, Guthrie DA, Støier JF, Werner C, Newman AH, Sauer M, Herborg F, et al.
Cell reports (2022) 4013: 111431. 113 011 ICC; tested species: rat
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy.
Richter KN, Revelo NH, Seitz KJ, Helm MS, Sarkar D, Saleeb RS, D'Este E, Eberle J, Wagner E, Vogl C, Lazaro DF, et al.
The EMBO journal (2018) 371: 139-159. 113 011 ICC; tested species: mouse
JIP3 localises to exocytic vesicles and focal adhesions in the growth cones of differentiated PC12 cells.
Caswell PT, Dickens M
Molecular and cellular biochemistry (2017) : . 113 011 ICC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 113 011 ICC; tested species: rat
A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis.
Ferguson SM, Brasnjo G, Hayashi M, Wölfel M, Collesi C, Giovedi S, Raimondi A, Gong LW, Ariel P, Paradise S, O'toole E, et al.
Science (New York, N.Y.) (2007) 3165824: 570-4. 113 011 WB, ICC
The first synapse in vision in the aging mouse retina.
Gierke K, Lux UT, Regus-Leidig H, Brandstätter JH
Frontiers in cellular neuroscience (2023) 17: 1291054. 113 011 IHC; tested species: mouse
Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson's disease.
Vidyadhara DJ, Somayaji M, Wade N, Yücel B, Zhao H, Shashaank N, Ribaudo J, Gupta J, Lam TT, Sames D, Greene LE, et al.
Cell reports (2023) 423: 112231. 113 011 WB, IHC; tested species: mouse
Modes and regulation of endocytic membrane retrieval in mouse auditory hair cells.
Neef J, Jung S, Wong AB, Reuter K, Pangrsic T, Chakrabarti R, Kügler S, Lenz C, Nouvian R, Boumil RM, Frankel WN, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 343: 705-16. 113 011 IHC; tested species: mouse
Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 113 011 IHC; tested species: rat
Synaptic pathology, altered gene expression, and degeneration in photoreceptors impacted by drusen.
Johnson PT, Brown MN, Pulliam BC, Anderson DH, Johnson LV
Investigative ophthalmology & visual science (2005) 4612: 4788-95. 113 011 IHC
The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina.
von Kriegstein K, Schmitz F
Cell and tissue research (2003) 3112: 159-73. 113 011 IHC
Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 113 011 WB, IHC
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 113 011 DNA-PAINT; tested species: rat
WB
ICC
IHC
IHC-P
Background
Clathrin consists of heavy chains and light chains that co-assemble to triskelions. The light chains are differentially spliced, with neurons expressing a special variant that is not detectable elsewhere. The neuron-specific light chains are enriched in synaptic nerve terminals where they participate in synaptic vesicle endocytosis.
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