Chromogranin B protein - 259-1P

Chromogranin B is expressed at high levels in the neuroendocrine system

control protein

Cat. No.: 259-1P

Amount: 100 µg

Price: $105.00

Cat. No. 259-1P	100 µg protein, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
Applications
Immunogen	Recombinant protein corresponding to AA 407 to 677 from mouse Chromogranin B (UniProt Id: P16014)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	259 103
Remarks	This control protein consists of the recombinant protein (aa 407-677 of mouse chromogranin B) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	259-1p.pdf

Cat. No.: 259-1P

Amount: 100 µg

Price: $105.00

Background

Chromogranin A (CgA) and B (CgB) are members of a family of acidic proteins stored and released throughout the neuroendocrine system. The large dense core vesicle associated proteins have multiple functions in neurons and neuroendocrine cells. They are differentially processed in different tissues.
Chromogranin A (CgA) is the precursor for the bioactive peptides pancreastatin, vasostatins, catestatin, β-granin and WE-14.

1 ) Domínguez N et al., J. Mol. Neurosci. (2012)
2 ) Park JJ et al., J. Cell. Sci. (2011)
3 ) Díaz-Vera J et al., J. Neurosci. (2010)
4 ) Schafer MK et al., Regul. Pept. (2010)
5 ) Machado JD et al., Cell. Mol. Neurobiol. (2010)
6 ) Montesinos MS et al., J. Neurosci. (2008)
7 ) Kim T et al., Cell (2001)
8 ) Natori S et al., EMBO J. (1998)
9 ) Kroesen S et al., Eur. J. Neurosci. (1996)
10 ) Wu HJ et al., J. Biol. Chem. (1991)

Protocols

OTHER

Preadsorbtion

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