Tailor-made Antibodies
and Tools for Life Science
Home|||||Technical Support

ChAT antibody - 297 015

ChAT is an enzyme involved in the synthesis of acetylcholine
Guinea pig polyclonal purified antibody
Cat. No.: 297 015
Amount: 50 µg
Price: $465.00
Cat. No. 297 015 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: 1 : 200 up to 1 : 500 gallery  
iDISCO: 1 : 400
Immunogen Recombinant protein corresponding to AA 2 to 640 from rat ChAT (UniProt Id: P32738)
Reactivity Reacts with: rat (P32738), mouse (Q03059), human (P28329).
Other species not tested yet.
Remarks

Replaces the crude Guinea pig antiserum cat. no. 297 014.

Data sheet 297_015.pdf

References for ChAT - 297 015

Ephrin-A3 is required for tonotopic map precision and auditory functions in the mouse auditory brainstem.
Hoshino N, Altarshan Y, Alzein A, Fernando AM, Nguyen HT, Majewski EF, Chen VC, Rochlin MW, Yu WM
The Journal of comparative neurology (2021) 52916: 3633-3654. 297 015 IHC; tested species: mouse
A CRHR1 antagonist prevents synaptic loss and memory deficits in a trauma-induced delirium-like syndrome.
Cursano S, Battaglia CR, Urrutia-Ruiz C, Grabrucker S, Schön M, Bockmann J, Braumüller S, Radermacher P, Roselli F, Huber-Lang M, Boeckers TM, et al.
Molecular psychiatry (2020) : . 297 015 IHC; tested species: mouse
Cat. No.: 297 015
Amount: 50 µg
Price: $465.00
Ephrin-A3 is required for tonotopic map precision and auditory functions in the mouse auditory brainstem.
Hoshino N, Altarshan Y, Alzein A, Fernando AM, Nguyen HT, Majewski EF, Chen VC, Rochlin MW, Yu WM
The Journal of comparative neurology (2021) 52916: 3633-3654. 297 015 IHC; tested species: mouse
A CRHR1 antagonist prevents synaptic loss and memory deficits in a trauma-induced delirium-like syndrome.
Cursano S, Battaglia CR, Urrutia-Ruiz C, Grabrucker S, Schön M, Bockmann J, Braumüller S, Radermacher P, Roselli F, Huber-Lang M, Boeckers TM, et al.
Molecular psychiatry (2020) : . 297 015 IHC; tested species: mouse
Background
Cholinergic neurons are involved in numerous neurological functions. Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons,postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells.
Two enzymes; choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) are involved in the metabolism of ACh.