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VAMP1/2/3 antibody - 104 011

Vamp1, 2 and 3 are major vesicle protein involved in fusion
Mouse monoclonal purified IgG
Cat. No.: 104 011
Amount: 100 µg
Price: $415.00
Cat. No. 104 011 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: 1 : 100 up to 1 : 500 gallery  
IHC-P: 1 : 500 up to 1 : 1000 gallery  
Clone 10.1
Subtype IgG1
Immunogen Recombinant protein corresponding to AA 1 to 118 from rat Synaptobrevin1 (UniProt Id: Q63666)
Epitop AA 31 to 98 from rat Synaptobrevin1 (UniProt Id: Q63666)
Reactivity Reacts with: human (P23763, P63027, Q15836), rat (Q63666, P63045, P63025), mouse (Q62442, P63044, P63024), vertebrates, invertebrates.
Other species not tested yet.
Specificity Detects the three isoforms VAMP 1, 2, 3.
Remarks

This antibody is not recommended for non-neuronal cells. Please use cat. no. 104 102 for such experiments.
This antibody reacts with the conserved middle-portion of the molecule. Its affinity/avidity is not very high, thus requiring extended incubation periods and sensitive detection systems.
WB: This antibody recognizes the Botulinumtoxin B cleavage product (aa 1 - 76) with reduced affinity. The sensitivity is sufficient for the detection of cleaved recombinant protein. For analysis of toxin treated tissue homogenates cat. no. 104 203 is recommended.

Data sheet 104_011.pdf

References for VAMP1/2/3 - 104 011

Maternal stress programs a demasculinization of glutamatergic transmission in stress-related brain regions of aged rats.
Verhaeghe R, Gao V, Morley-Fletcher S, Bouwalerh H, Van Camp G, Cisani F, Nicoletti F, Maccari S
GeroScience (2021) : . 104 011 WB; tested species: rat
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 104 011 WB, ICC
A common mechanism for the regulation of vesicular SNAREs on phospholipid membranes.
Hu K, Rickman C, Carroll J, Davletov B
The Biochemical journal (2004) 377Pt 3: 781-5. 104 011 WB
A role for Sec1/Munc18 proteins in platelet exocytosis.
Schraw TD, Lemons PP, Dean WL, Whiteheart SW
The Biochemical journal (2003) 374Pt 1: 207-17. 104 011 WB; tested species: human
Homozygous STXBP1 variant causes encephalopathy and gain-of-function in synaptic transmission.
Lammertse HCA, van Berkel AA, Iacomino M, Toonen RF, Striano P, Gambardella A, Verhage M, Zara F
Brain : a journal of neurology (2019) : . 104 011 ICC; tested species: mouse
VAPB depletion alters neuritogenesis and phosphoinositide balance in motoneuron-like cells: relevance to VAPB-linked ALS.
Genevini P, Colombo MN, Venditti R, Marcuzzo S, Colombo SF, Bernasconi P, De Matteis MA, Borgese N, Navone F
Journal of cell science (2019) : . 104 011 ICC; tested species: mouse
Hsc70 chaperone activity is required for the cytosolic slow axonal transport of synapsin.
Ganguly A, Han X, Das U, Wang L, Loi J, Sun J, Gitler D, Caillol G, Leterrier C, Yates JR, Roy S, et al.
The Journal of cell biology (2017) 2167: 2059-2074. 104 011 ICC; tested species: mouse
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 104 011 WB, ICC
The zebrafish pinball wizard gene encodes WRB, a tail-anchored-protein receptor essential for inner-ear hair cells and retinal photoreceptors.
Lin SY, Vollrath MA, Mangosing S, Shen J, Cardenas E, Corey DP
The Journal of physiology (2016) 5944: 895-914. 104 011 IHC; tested species: zebrafish
Synaptotagmin I, synaptobrevin II, and syntaxin I are coexpressed in rat and gerbil pinealocytes.
Redecker P
Cell and tissue research (1996) 2833: 443-54. 104 011 IHC; tested species: rat
Cat. No.: 104 011
Amount: 100 µg
Price: $415.00
Maternal stress programs a demasculinization of glutamatergic transmission in stress-related brain regions of aged rats.
Verhaeghe R, Gao V, Morley-Fletcher S, Bouwalerh H, Van Camp G, Cisani F, Nicoletti F, Maccari S
GeroScience (2021) : . 104 011 WB; tested species: rat
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 104 011 WB, ICC
A common mechanism for the regulation of vesicular SNAREs on phospholipid membranes.
Hu K, Rickman C, Carroll J, Davletov B
The Biochemical journal (2004) 377Pt 3: 781-5. 104 011 WB
A role for Sec1/Munc18 proteins in platelet exocytosis.
Schraw TD, Lemons PP, Dean WL, Whiteheart SW
The Biochemical journal (2003) 374Pt 1: 207-17. 104 011 WB; tested species: human
Homozygous STXBP1 variant causes encephalopathy and gain-of-function in synaptic transmission.
Lammertse HCA, van Berkel AA, Iacomino M, Toonen RF, Striano P, Gambardella A, Verhage M, Zara F
Brain : a journal of neurology (2019) : . 104 011 ICC; tested species: mouse
VAPB depletion alters neuritogenesis and phosphoinositide balance in motoneuron-like cells: relevance to VAPB-linked ALS.
Genevini P, Colombo MN, Venditti R, Marcuzzo S, Colombo SF, Bernasconi P, De Matteis MA, Borgese N, Navone F
Journal of cell science (2019) : . 104 011 ICC; tested species: mouse
Hsc70 chaperone activity is required for the cytosolic slow axonal transport of synapsin.
Ganguly A, Han X, Das U, Wang L, Loi J, Sun J, Gitler D, Caillol G, Leterrier C, Yates JR, Roy S, et al.
The Journal of cell biology (2017) 2167: 2059-2074. 104 011 ICC; tested species: mouse
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 104 011 WB, ICC
The zebrafish pinball wizard gene encodes WRB, a tail-anchored-protein receptor essential for inner-ear hair cells and retinal photoreceptors.
Lin SY, Vollrath MA, Mangosing S, Shen J, Cardenas E, Corey DP
The Journal of physiology (2016) 5944: 895-914. 104 011 IHC; tested species: zebrafish
Synaptotagmin I, synaptobrevin II, and syntaxin I are coexpressed in rat and gerbil pinealocytes.
Redecker P
Cell and tissue research (1996) 2833: 443-54. 104 011 IHC; tested species: rat
Background

Synaptobrevins/VAMPs represents a family of integral membrane proteins of 11-13 kDa with the N-terminal region exposed to the cytoplasm and a C-terminal transmembrane domain. Two isoforms were identified in the mammalian CNS, synaptobrevin 1 (VAMP 1 or p18-1) and synaptobrevin 2 (VAMP 2 or p18-2) that differ in their distribution within different brain regions.
Synaptobrevin 1 is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin 2 are present in many other tissues where the protein resides on specialized microvesicles.
In non-neuronal cells the third isoform, cellubrevin (VAMP 3), is present where it is localized to an endosomal membrane pool.
Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v-SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C-terminal portion of the molecule.