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Home > Products > 371 011C2

CD63 antibody - 371 011C2

CD63 is a lysosome associated membrane protein
Mouse monoclonal purified IgG
Cat. No.: 371 011C2
Amount: 100 µg
Price: $465.00
Cat. No. 371 011C2 100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 2.

Fluorescence labeled antibodies conjugated to Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

Cyanine 2: λex 492 nm / λem 508 nm
Sulfo-Cyanine 3: λex 554 nm / λem 568 nm
Sulfo-Cyanine 5: λex 649 nm / λem 667 nm

Cyanine 5 dyes are well suited for STORM high resolution microscopy.
Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates.

 Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: not recommended
IP: N/A
ICC: 1 : 250 up to 1 : 1000 gallery  
IHC: not tested yet
IHC-P: not tested yet
FACS: yes
Label Cyanine 2
Clone 101G9
Subtype IgG2b (κ light chain)
Immunogen Purified human basophil granulocytes. (UniProt Id: P08962)
Epitop AA 103 to 203 from human CD63 (UniProt Id: P08962)
Reactivity Reacts with: human (P08962).
Other species not tested yet.
Data sheet 371_011c2.pdf
Cat. No.: 371 011C2
Amount: 100 µg
Price: $465.00
ICC
ICC
Background
CD 63 is a glycoprotein with four transmembrane domains. It is expressed in several cell types and localizes to late endosomes, lysosomes and secretory vesicles. In basophile granulocytes it is transiently exposed on the cell surface after activation. CD 63 expression is also associated with early stages of melanoma tumor progression.
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