CD63 antibody - 371 011C2

CD63 is a lysosome associated membrane protein

Mouse monoclonal purified IgG

Cat. No.: 371 011C2

Amount: 100 µg

Price: $465.00

Cat. No. 371 011C2	100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 2. Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy. Cyanine 2: λ_ex 492 nm / λ_em 508 nm Sulfo-Cyanine 3: λ_ex 554 nm / λ_em 568 nm Sulfo-Cyanine 5: λ_ex 649 nm / λ_em 667 nm (Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy. Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates. Albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: not recommended IP: N/A ICC: 1 : 250 up to 1 : 1000 gallery IHC: not tested yet IHC-P: not tested yet FACS: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.
Label	Cyanine 2
Clone	101G9
Subtype	IgG2b (κ light chain)
Immunogen	Purified human basophil granulocytes. (UniProt Id: P08962)
Epitop	AA 103 to 203 from human CD63 (UniProt Id: P08962)
Reactivity	Reacts with: human (P08962). Other species not tested yet.
Data sheet	371_011c2.pdf

Cat. No.: 371 011C2

Amount: 100 µg

Price: $465.00

ICC

Background

CD 63 is a glycoprotein with four transmembrane domains. It is expressed in several cell types and localizes to late endosomes, lysosomes and secretory vesicles. In basophile granulocytes it is transiently exposed on the cell surface after activation. CD 63 expression is also associated with early stages of melanoma tumor progression.

1 ) Grünwald B et al., Gastroenterology (2016)
2 ) Lupia A et al., J. Invest. Dermatol. (2014)
3 ) Kraft S et al., J. Immunol. (2013)
4 ) Tammaro A et al., Int J Immunopathol Pharmacol ()
5 ) Moneret-Vautrin DA et al., Ann. Allergy Asthma Immunol. (1999)
6 ) Metzelaar MJ et al., J. Biol. Chem. (1991)

Protocols

ICC

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