CD63 antibody - 371 011

CD63 is a lysosome associated membrane protein

Mouse monoclonal purified IgG

Cat. No.: 371 011

Amount: 100 µg

Price: $415.00

Cat. No. 371 011	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 250 up to 1 : 1000 gallery IHC: not tested yet IHC-P: 1 : 1000 up to 1 : 5000 gallery FACS: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.
Clone	101G9
Subtype	IgG2b (κ light chain)
Immunogen	Purified human basophil granulocytes. (UniProt Id: P08962)
Epitop	AA 103 to 203 from human CD63 (UniProt Id: P08962)
Reactivity	Reacts with: human (P08962). Other species not tested yet.
Data sheet	371_011.pdf

Cat. No.: 371 011

Amount: 100 µg

Price: $415.00

ICC

IHC-P

Background

CD 63 is a glycoprotein with four transmembrane domains. It is expressed in several cell types and localizes to late endosomes, lysosomes and secretory vesicles. In basophile granulocytes it is transiently exposed on the cell surface after activation. CD 63 expression is also associated with early stages of melanoma tumor progression.

1 ) Grünwald B et al., Gastroenterology (2016)
2 ) Lupia A et al., J. Invest. Dermatol. (2014)
3 ) Kraft S et al., J. Immunol. (2013)
4 ) Tammaro A et al., Int J Immunopathol Pharmacol ()
5 ) Moneret-Vautrin DA et al., Ann. Allergy Asthma Immunol. (1999)
6 ) Metzelaar MJ et al., J. Biol. Chem. (1991)

Protocols

ICC

IHC-P

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