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CD34 antibody - 370 011

CD34 is a hematopoietic progenitor cell antigen
Mouse monoclonal purified IgG
Cat. No.: 370 011
Amount: 100 µg
Price: $415.00
Cat. No. 370 011 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: not recommended
FACS: yes

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.

Clone 171G9
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 32 to 291 from human CD34 (UniProt Id: P28906)
Reactivity Reacts with: human (P28906).
Other species not tested yet.
Data sheet 370_011.pdf
Cat. No.: 370 011
Amount: 100 µg
Price: $415.00
Background
CD 34 is a single pass trans-membrane glycoprotein that localizes to the surface of hematopoetic progenitor cells. The presence of CD 34 on non-hematopoietic cells in various tissues has been linked to progenitor and adult stem cell phenotypes.