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Home > Products > 258 011

Cannabinoid receptor CB1-R antibody - 258 011 K.O.

CB1-R is a G-protein coupled receptor responsive to cannabinoids
Mouse monoclonal purified IgG
Cat. No.: 258 011
Amount: 100 µg
Price: $420.00
Cat. No. 258 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 289C1
Subtype IgG2b (κ light chain)
Immunogen Synthetic peptide corresponding to AA 450 to 473 from rat CB1-R (UniProt Id: P20272)
Reactivity Reacts with: rat (P20272), mouse (P47746), human (P21554).
Other species not tested yet.
Specificity K.O. validated
Data sheet 258_011.pdf

References for Cannabinoid receptor CB1-R - 258 011

Impact of the mouse estrus cycle on cannabinoid receptor agonist-induced molecular and behavioral outcomes.
Kim HJJ, Zagzoog A, Black T, Baccetto SL, Ezeaka UC, Laprairie RB
Pharmacology research & perspectives (2022) 103: e00950. 258 011 WB; tested species: mouse
Cannabidiol modulates excitatory-inhibitory ratio to counter hippocampal hyperactivity.
Rosenberg EC, Chamberland S, Bazelot M, Nebet ER, Wang X, McKenzie S, Jain S, Greenhill S, Wilson M, Marley N, Salah A, et al.
Neuron (2023) : . 258 011 ICC, IHC; tested species: mouse,rat
CB1R blockade unmasks TRPV1-mediated contextual fear generalization in female, but not male rats.
Huckleberry KA, Calitri R, Li AJ, Mejdell M, Singh A, Bhutani V, Laine MA, Nastase AS, Morena M, Hill MN, Shansky RM, et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2023) 4810: 1500-1508. 258 011 IHC; tested species: rat
Cannabidiol modulates excitatory-inhibitory ratio to counter hippocampal hyperactivity.
Rosenberg EC, Chamberland S, Bazelot M, Nebet ER, Wang X, McKenzie S, Jain S, Greenhill S, Wilson M, Marley N, Salah A, et al.
Neuron (2023) : . 258 011 ICC, IHC; tested species: mouse,rat
Dual Cannabinoid and Orexin Regulation of Anhedonic Behaviour Caused by Prolonged Restraint Stress.
Kim HJJ, Zagzoog A, Ceni C, Ferrisi R, Janz N, Laprairie RB
Brain sciences (2023) 132: . 258 011 IHC; tested species: mouse
Social Play Behavior Is Critical for the Development of Prefrontal Inhibitory Synapses and Cognitive Flexibility in Rats.
Bijlsma A, Omrani A, Spoelder M, Verharen JPH, Bauer L, Cornelis C, de Zwart B, van Dorland R, Vanderschuren LJMJ, Wierenga CJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4246: 8716-8728. 258 011 IHC; tested species: rat
Deficiency in endocannabinoid synthase DAGLB contributes to early onset Parkinsonism and murine nigral dopaminergic neuron dysfunction.
Liu Z, Yang N, Dong J, Tian W, Chang L, Ma J, Guo J, Tan J, Dong A, He K, Zhou J, et al.
Nature communications (2022) 131: 3490. 258 011 IHC; tested species: mouse
Axonal CB1 Receptors Mediate Inhibitory Bouton Formation via cAMP Increase and PKA.
Liang J, Kruijssen DLH, Verschuuren ACJ, Voesenek BJB, Benavides FFW, Sáez Gonzalez M, Ruiter M, Wierenga CJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4140: 8279-8296. 258 011 IHC; tested species: mouse
In vivo Evidence for Brain Region-Specific Molecular Interactions Between Cannabinoid and Orexin Receptors.
Kim HJJ, Zagzoog A, Smolyakova AM, Ezeaka UC, Benko MJ, Holt T, Laprairie RB
Frontiers in neuroscience (2021) 15: 790546. 258 011 IHC; tested species: mouse
Effects of ∆9-THC on memory in ovariectomized and intact female rats.
DeLarge AF, Winsauer PJ
Hormones and behavior (2021) 127: 104883. 258 011 IHC; tested species: rat
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 258 011 IHC; tested species: rat
Early-life stress exacerbates the effects of WIN55,212-2 and modulates the cannabinoid receptor type 1 expression.
Martín-Sánchez A, García-Baos A, Castro-Zavala A, Alegre-Zurano L, Valverde O
Neuropharmacology (2020) : 108416. 258 011 IHC; tested species: mouse
Endocannabinoid Signaling Mediates Local Dendritic Coordination between Excitatory and Inhibitory Synapses.
Hu HY, Kruijssen DLH, Frias CP, Rózsa B, Hoogenraad CC, Wierenga CJ
Cell reports (2019) 273: 666-675.e5. 258 011 IHC; tested species: mouse
The cannabinoid-1 receptor is abundantly expressed in striatal striosomes and striosome-dendron bouquets of the substantia nigra.
Davis MI, Crittenden JR, Feng AY, Kupferschmidt DA, Naydenov A, Stella N, Graybiel AM, Lovinger DM
PloS one (2018) 132: e0191436. 258 011 IHC; tested species: mouse
Cat. No.: 258 011
Amount: 100 µg
Price: $420.00
Impact of the mouse estrus cycle on cannabinoid receptor agonist-induced molecular and behavioral outcomes.
Kim HJJ, Zagzoog A, Black T, Baccetto SL, Ezeaka UC, Laprairie RB
Pharmacology research & perspectives (2022) 103: e00950. 258 011 WB; tested species: mouse
Cannabidiol modulates excitatory-inhibitory ratio to counter hippocampal hyperactivity.
Rosenberg EC, Chamberland S, Bazelot M, Nebet ER, Wang X, McKenzie S, Jain S, Greenhill S, Wilson M, Marley N, Salah A, et al.
Neuron (2023) : . 258 011 ICC, IHC; tested species: mouse,rat
CB1R blockade unmasks TRPV1-mediated contextual fear generalization in female, but not male rats.
Huckleberry KA, Calitri R, Li AJ, Mejdell M, Singh A, Bhutani V, Laine MA, Nastase AS, Morena M, Hill MN, Shansky RM, et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2023) 4810: 1500-1508. 258 011 IHC; tested species: rat
Cannabidiol modulates excitatory-inhibitory ratio to counter hippocampal hyperactivity.
Rosenberg EC, Chamberland S, Bazelot M, Nebet ER, Wang X, McKenzie S, Jain S, Greenhill S, Wilson M, Marley N, Salah A, et al.
Neuron (2023) : . 258 011 ICC, IHC; tested species: mouse,rat
Dual Cannabinoid and Orexin Regulation of Anhedonic Behaviour Caused by Prolonged Restraint Stress.
Kim HJJ, Zagzoog A, Ceni C, Ferrisi R, Janz N, Laprairie RB
Brain sciences (2023) 132: . 258 011 IHC; tested species: mouse
Social Play Behavior Is Critical for the Development of Prefrontal Inhibitory Synapses and Cognitive Flexibility in Rats.
Bijlsma A, Omrani A, Spoelder M, Verharen JPH, Bauer L, Cornelis C, de Zwart B, van Dorland R, Vanderschuren LJMJ, Wierenga CJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4246: 8716-8728. 258 011 IHC; tested species: rat
Deficiency in endocannabinoid synthase DAGLB contributes to early onset Parkinsonism and murine nigral dopaminergic neuron dysfunction.
Liu Z, Yang N, Dong J, Tian W, Chang L, Ma J, Guo J, Tan J, Dong A, He K, Zhou J, et al.
Nature communications (2022) 131: 3490. 258 011 IHC; tested species: mouse
Axonal CB1 Receptors Mediate Inhibitory Bouton Formation via cAMP Increase and PKA.
Liang J, Kruijssen DLH, Verschuuren ACJ, Voesenek BJB, Benavides FFW, Sáez Gonzalez M, Ruiter M, Wierenga CJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4140: 8279-8296. 258 011 IHC; tested species: mouse
In vivo Evidence for Brain Region-Specific Molecular Interactions Between Cannabinoid and Orexin Receptors.
Kim HJJ, Zagzoog A, Smolyakova AM, Ezeaka UC, Benko MJ, Holt T, Laprairie RB
Frontiers in neuroscience (2021) 15: 790546. 258 011 IHC; tested species: mouse
Effects of ∆9-THC on memory in ovariectomized and intact female rats.
DeLarge AF, Winsauer PJ
Hormones and behavior (2021) 127: 104883. 258 011 IHC; tested species: rat
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 258 011 IHC; tested species: rat
Early-life stress exacerbates the effects of WIN55,212-2 and modulates the cannabinoid receptor type 1 expression.
Martín-Sánchez A, García-Baos A, Castro-Zavala A, Alegre-Zurano L, Valverde O
Neuropharmacology (2020) : 108416. 258 011 IHC; tested species: mouse
Endocannabinoid Signaling Mediates Local Dendritic Coordination between Excitatory and Inhibitory Synapses.
Hu HY, Kruijssen DLH, Frias CP, Rózsa B, Hoogenraad CC, Wierenga CJ
Cell reports (2019) 273: 666-675.e5. 258 011 IHC; tested species: mouse
The cannabinoid-1 receptor is abundantly expressed in striatal striosomes and striosome-dendron bouquets of the substantia nigra.
Davis MI, Crittenden JR, Feng AY, Kupferschmidt DA, Naydenov A, Stella N, Graybiel AM, Lovinger DM
PloS one (2018) 132: e0191436. 258 011 IHC; tested species: mouse
ICC
IHC
IHC
IHC-P
Background
The cannabinoid receptor CB1-R is a G-protein coupled receptor (GPCR) with 7 transmembrane domains. It is responsive to tetrahydrocannabinol, the psychotropic component of marijuana.
Endogenous cannabinoids (endocannabinoids) are released from postsynaptic neurons and act onto presynaptic cannabinoid receptors where they play important physiological roles in synaptic plasticity, analgesia, appetite, and neuroprotection.
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