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CASKIN1 antibody - 185 003 K.O.

CASKIN is a CASK interaction partner
Rabbit polyclonal purified antibody
Cat. No.: 185 003
Amount: 50 µg
Price: $455.00
Cat. No. 185 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 200 gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Synthetic peptide corresponding to AA 1416 to 1430 from rat CASKIN1 (UniProt Id: Q8VHK2)
Specificity Specific for CASKIN 1.The epiotope is present in splice variants 1, 2, 3 but missing in variant 4. K.O. validated PubMed: 31727973
Matching control protein/peptide 185-0P
Data sheet 185_003.pdf

References for CASKIN1 - 185 003

Presynaptic PTPσ regulates postsynaptic NMDA receptor function through direct adhesion-independent mechanisms.
Kim K, Shin W, Kang M, Lee S, Kim D, Kang R, Jung Y, Cho Y, Yang E, Kim H, Bae YC, et al.
eLife (2020) 9: . 185 003 WB; tested species: mouse
Dendritic spine morphology and memory formation depend on postsynaptic Caskin proteins.
Bencsik N, Pusztai S, Borbély S, Fekete A, Dülk M, Kis V, Pesti S, Vas V, Szűcs A, Buday L, Schlett K, et al.
Scientific reports (2019) 91: 16843. 185 003 WB; KO verified; tested species: mouse
Cat. No.: 185 003
Amount: 50 µg
Price: $455.00
Presynaptic PTPσ regulates postsynaptic NMDA receptor function through direct adhesion-independent mechanisms.
Kim K, Shin W, Kang M, Lee S, Kim D, Kang R, Jung Y, Cho Y, Yang E, Kim H, Bae YC, et al.
eLife (2020) 9: . 185 003 WB; tested species: mouse
Dendritic spine morphology and memory formation depend on postsynaptic Caskin proteins.
Bencsik N, Pusztai S, Borbély S, Fekete A, Dülk M, Kis V, Pesti S, Vas V, Szűcs A, Buday L, Schlett K, et al.
Scientific reports (2019) 91: 16843. 185 003 WB; KO verified; tested species: mouse
Background

The complex of the multi-adaptor-proteins CASK, Veli and Mint is conserved across kingdoms. CASKIN 1 is a novel CASK interaction partner that binds to its Cam Kinase domain and competes with Mint so that alternative complexes can be formed. Four different splice variants have been identified so far.